Qualitative identification of Chinese medicine Bairui tablet and method for measuring kaempferol content therein

Abstract

The invention discloses qualitative identification of traditional Chinese medicine thesium Chinese tablets and a method for measuring the content of kaempferia galangal in the thesium Chinese tablets. Qualitative identification of the thesium Chinese tablets is carried out by adopting a thin layer chromatogram, the method for measuring the content of the kaempferia galangal in the thesium Chinese tablets by utilizing high performance liquid chromatography is established, and the weight of the kaempferia galangal in each thesium Chinese tablet ranges from 0.57 mg to 0.61 mg. The method can effectively control the mass of the thesium Chinese tablets and has a great practical value.

Description

technical field

[0001] The invention belongs to a traditional Chinese medicine detection method, and specifically relates to a qualitative identification of traditional Chinese medicine Bairui tablets and a method for measuring the content of kaempferol therein. Background technique:

[0002] Bai Rui Tablet is a Chinese patent medicine composed of Bai Rui herbs and medicinal dressings. Clinically, it is commonly used for acute and chronic pharyngitis, bronchitis, rhinitis, cold and fever, pneumonia, etc. In the quality control of Bairui tablets, only the total flavonoids were identified by color reaction, and other quality problems were not detected. Therefore, the original quality control method could not reflect the intrinsic quality of the drug, and the produced Bairui tablets It is difficult to ensure the quality of the medicine, limiting its use. As a result, there will be some adverse consequences, which will not only affect the reputation of the product, but also af...

Examples

Embodiment 1

[0092] Example 1 Thin-layer identification of baishi sheet

[0093] Preparation of the test solution: take 5 pieces of each of the three batches (060201, 060202, 060203), remove the sugar coating, grind finely, add 20 ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 2 ml of methanol to dissolve, as the test solution.

[0094] Preparation of blank sample solution: take 2 g of blank sample and prepare in the same way.

[0095] Preparation of the reference medicinal material solution: take 0.5 g of the reference medicinal material of balsam pear, add 10 ml of methanol, and prepare by the same method.

[0096] Thin-layer plate: silica gel G thin-layer plate; developing agent: n-butanol-acetic acid-water (4:1:5) upper layer solution; Spotting volume: control medicinal material solution, test solution, blank solution each 5μl, unfold, air Dry, viewed at 365 nm.

[0097] Result: In the chromatogram of the test substance, there are...

example 2

[0098] Example 2 Determination of kaempferol content in Bairui tablets

[0099] batch number Kaempferol (mg / tablet) 060201 0.61 060202 0.57 060203 0.58 average 0.59

[0100] (1) Preparation of reference substance solution: Take kaempferol reference substance, add methanol to make a solution containing 30 μg per 1ml, and use it as a reference substance solution.

[0101] (2) Preparation of the test solution: take 5 tablets of this product, remove the sugar coating, grind it finely, weigh 1 g, accurately weigh it, add 30 ml of 70% ethanol, 5 ml of 25% hydrochloric acid, and hydrolyze at 85°C-90°C for 60 minutes. Take out, cool rapidly, and dissolve to 50ml with 70% ethanol. Filtration, take the continued filtrate, that is.

[0102] (3) Chromatographic conditions and system suitability test: use octadecylsilane-bonded silica gel as filler, use methanol-0.5% phosphoric acid solution (50:50) V / V as mobile phase; flow rate is 1ml / min; detection...

technical field

[0001] The invention belongs to a traditional Chinese medicine detection method, and specifically relates to a qualitative identification of traditional Chinese medicine Bairui tablets and a method for measuring the content of kaempferol therein. Background technique:

[0002] Bai Rui Tablet is a Chinese patent medicine composed of Bai Rui herbs and medicinal dressings. Clinically, it is commonly used for acute and chronic pharyngitis, bronchitis, rhinitis, cold and fever, pneumonia, etc. In the quality control of Bairui tablets, only the total flavonoids were identified by color reaction, and other quality problems were not detected. Therefore, the original quality control method could not reflect the intrinsic quality of the drug, and the produced Bairui tablets It is difficult to ensure the quality of the medicine, limiting its use. As a result, there will be some adverse consequences, which will not only affect the reputation of the product, but also af...

Examples

Embodiment 1

[0092] Example 1 Thin-layer identification of baishi sheet

[0093] Preparation of the test solution: take 5 pieces of each of the three batches (060201, 060202, 060203), remove the sugar coating, grind finely, add 20 ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 2 ml of methanol to dissolve, as the test solution.

[0094] Preparation of blank sample solution: take 2 g of blank sample and prepare in the same way.

[0095] Preparation of the reference medicinal material solution: take 0.5 g of the reference medicinal material of balsam pear, add 10 ml of methanol, and prepare by the same method.

[0096] Thin-layer plate: silica gel G thin-layer plate; developing agent: n-butanol-acetic acid-water (4:1:5) upper layer solution; Spotting volume: control medicinal material solution, test solution, blank solution each 5μl, unfold, air Dry, viewed at 365 nm.

[0097] Result: In the chromatogram of the test substance, there are...

example 2

[0098] Example 2 Determination of kaempferol content in Bairui tablets

[0099] batch number Kaempferol (mg / tablet) 060201 0.61 060202 0.57 060203 0.58 average 0.59

[0100] (1) Preparation of reference substance solution: Take kaempferol reference substance, add methanol to make a solution containing 30 μg per 1ml, and use it as a reference substance solution.

[0101] (2) Preparation of the test solution: take 5 tablets of this product, remove the sugar coating, grind it finely, weigh 1 g, accurately weigh it, add 30 ml of 70% ethanol, 5 ml of 25% hydrochloric acid, and hydrolyze at 85°C-90°C for 60 minutes. Take out, cool rapidly, and dissolve to 50ml with 70% ethanol. Filtration, take the continued filtrate, that is.

[0102] (3) Chromatographic conditions and system suitability test: use octadecylsilane-bonded silica gel as filler, use methanol-0.5% phosphoric acid solution (50:50) V / V as mobile phase; flow rate is 1ml / min; detection...

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