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Method for preparing fibrinolysis enzyme

A technology of fibrinolysis and peptone, applied in the field of food biology, can solve the problems of high price, shortage of sources, and difficulty in exerting drug effects, and achieve the effect of low cost and strong feasibility

Inactive Publication Date: 2009-06-10
J JIANGSU LIANGFENG FOODSTUFF GROUP
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AI Technical Summary

Problems solved by technology

The research and development of thrombolytic agents are quite active, but the thrombolytic drugs used in clinical practice have certain defects to varying degrees; or they are highly toxic and have large side effects; shortage, expensive

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0014] Bacillus is fermented under the following conditions,

[0015] Seed culture: seed culture medium (by mass volume percentage) is: peptone 1%, glucose 1%, yeast extract 0.5%, Na 2 HPO 4 12H2O 1.2%, MgSO 4 7H 2 O 0.03%, adjusted to pH 7.2, sterilized at 121°C for 20 minutes, culture conditions: shaker speed 180rpm, culture temperature 37°C, culture time 16-18 hours;

[0016] Liquid fermentation culture: liquid fermentation medium (in terms of mass volume percentage) is: peptone 1.5%, glucose 1.5%, Na 2 HPO 4 12H 2 O 1.5%, NaH 2 PO 4 2H 2 O 0.13%, MgSO 4 7H 2 O 0.1%, defoamer polyoxyethylene oxypropylene triglyceride 0.03%, sterilized at 121°C for 20 minutes, and adjusted to pH 7.2; the seed culture medium was inserted into 15 liters of liquid fermentation medium by volume percentage 5% In a 25-liter fermenter, the culture conditions are: stirring speed 180rpm, control ventilation: 9L / min for 0-12 hours, 10.5L / min for 12-24 hours, 12L / min for 24-36 hours; co...

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Abstract

The invention discloses a method for preparing fibrinolytic enzyme. The method comprises the steps of utilizing fibrinolytic enzyme strains, namely bacillus to produce the fibrinolytic enzyme and performing liquid fermentation through bacteria. The method is low in production cost and strong in feasibility, and can be used for mass production. The fibrinolytic enzyme is directly made into capsules, medicinal granules, oral liquid or oral buccal tablets, or is used as functional food ingredient in health-care food or common food. The method improves the prior method of screening fibrinolytic-enzyme producing strain, adopts a liquid medium for enrichment culture and a screening method with panel primary screening which takes fibrin as main matrix and shake-flask secondary screening, and simultaneously determines fibrinolytic activity. The enzyme activity of obtained fermentation broth is higher than 588.4 u / ml.

Description

technical field [0001] The invention relates to a preparation method for producing fibrinolytic enzyme mainly by using the fibrinolytic enzyme strain-Bacillus, belonging to the field of food biotechnology. Background technique [0002] Cardiovascular and cerebrovascular diseases have always been known as the number one killer that threatens human health. According to relevant survey reports, more than 3 million people die of cardiovascular and cerebrovascular diseases in my country every year, accounting for 50% of the total annual death toll in my country. Among them, 75% are disabled to varying degrees, and 4% are seriously disabled. Thromboembolic disease is the first cause of death and disability. Without thrombosis, there will be no cardiovascular and cerebrovascular events. [0003] Clinically, there are three commonly used treatment methods for thromboembolic diseases: first, surgery, which is the most rapid and direct method to remove thrombus. This method has compl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/68
Inventor 蔡健季万兰
Owner J JIANGSU LIANGFENG FOODSTUFF GROUP
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