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Soil bacterium screening method

A screening method and bacterial technology, applied to the separation of microorganisms, etc., can solve the problems of long screening time, unfavorable bacterial diversification experimental research, and unsatisfactory experiments, etc., to improve bacterial screening efficiency, improve bacterial screening methods, and simple bacterial screening Effect

Inactive Publication Date: 2018-09-07
郭庆
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the screening method of bacteria, the selection medium is used to screen the bacteria, and the bacteria are screened by the size of the bacteria. The selection medium is used to screen the bacteria. The screening accuracy is high, but the screening takes a long time, which is not conducive to the rapid screening of bacteria. The screening efficiency is low, and when there is a large difference in the volume of bacteria, the bacteria can be centrifuged according to the size of the bacteria, and the desired bacteria can be quickly screened out. However, many bacteria separation devices have only one centrifugal net, and the pore size of the centrifugal net remains unchanged. It cannot be adjusted, and each bacteria separation device can only screen bacteria of the same particle size. The bacteria separation device cannot perform multi-stage separation of bacteria, and cannot meet the requirements for the separation of bacteria of various sizes and different sizes, and cannot meet the needs of experiments. , not conducive to the experimental study of bacterial diversification

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0049] Embodiment one: by changing the magnetic size of the electromagnet 323, the greater the magnetism of the electromagnet 323, the closer the magnetotactic bacteria is to the electromagnet 323, the inner diameter of the elastic bag 322 will reduce, and the aperture of the sieve hole 321 will reduce. By controlling The magnetism of the electromagnet 323 controls the aperture size of the sieve hole 321, and then controls the cylindrical sieve 32 to screen bacteria of different sizes.

Embodiment 2

[0050] Embodiment 2: By changing the light intensity of the lighting lamp 324, the greater the light intensity, the closer the phototactic bacteria are to the lighting lamp 324, the inner diameter of the elastic capsule 322 will be reduced, and the aperture of the sieve hole 321 will be reduced. By controlling the lighting lamp 324 The intensity of light is used to control the aperture size of the sieve 321, and then control the barrel-shaped sieve 32 to screen bacteria of different sizes.

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Abstract

The invention belongs to the technical field of bacterium screening and in particular relates to a soil bacterium screening method. The screening method adopts a screening system; the screening systemcomprises a body, a filtering module, a screening module, a bacterium receiving module and a triggering type valve, wherein the body is provided with a bacterium temporary storage chamber; the filtering module is located at the upper end of the screening module; the filtering module is used for filtering grain residues in bacterium liquid; the screening module is located in the bacterium temporary storage chamber; the screening module is used for screening bacteria with different sizes; the bacterium receiving module is located at the lower end of the screening module; the bacterium receivingmodule is used for receiving bacteria screened by the screening module; the triggering type valve comprises a left half part and a right half part; the left half part is fixed on the bacterium receiving module and the right half part is fixed at the lower part of a hard liquid leakage pipe; the triggering type valve is used for controlling the bacteria screened by the screening module to accurately enter the bacterium receiving module. According to the soil bacterium screening method provided by the invention, the bacteria with different sizes can be screened and stored respectively in sequence, so that the bacteria are simpler and more convenient to screen and the bacterium screening efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of bacteria screening, in particular to a soil bacteria screening method. Background technique [0002] Soil bacteria are the most numerous and widely distributed among soil microorganisms. Autotrophic bacteria can directly use light energy or the energy released by the oxidation of inorganic substances, and can assimilate carbon dioxide for nutrition, such as nitrifying bacteria, sulfur bacteria, sulfur bacteria, iron bacteria, and hydrogen bacteria. Heterotrophic bacteria obtain energy and carbon sources from organic matter. For agricultural production, soil bacteria can be divided into beneficial and harmful. The beneficial ones include nitrogen-fixing bacteria, nitrifying bacteria and saprophytic bacteria, and the harmful ones include denitrifying bacteria. The research on soil bacteria has practical value and is conducive to improving soil The degree of fertility can increase the yield of crops, so th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
CPCC12N1/02
Inventor 郭庆张瑞
Owner 郭庆
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