Biological load indicator and method of measuring biological load

A technology of indicators and growth factors, applied in biological testing, biomaterial analysis, measuring devices, etc., can solve problems such as the presence or absence of stress or the lack of clear indicators of strength

Active Publication Date: 2009-06-10
关山敦生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, there are individual differences in the perception of stress, and there are no clear indicators of the presence or intensity of stress

Method used

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  • Biological load indicator and method of measuring biological load
  • Biological load indicator and method of measuring biological load
  • Biological load indicator and method of measuring biological load

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0214] Example 1: Scale of Mental State and Fatigue State

[0215] A questionnaire psychological test was conducted on 402 volunteer subjects, using SDS, MAS, GHQ, STAI, CMI, subjective symptom survey (produced by the Labor Health Research Institute), social terror scale, crowd terror scale and independently developed living conditions questionnaire Conduct an investigation to confirm that the subject is healthy. After confirming the health of the subject, 1ml of blood was taken at the same time point on the morning of the rest day and the morning of the end of the night shift, respectively, and put into the blood collection base cone (spitz) containing anticoagulant, and cooled at 4°C Plasma was separated under conditions. Plasma was stored frozen or on ice, and the respective cytokines were measured simultaneously using a microbead protein array system (Bio-Plex Suspension Array System manufactured by Bio-Rad Laboratory independently modified by the present inventors). C...

Embodiment 2

[0217] Example 2: Scale of Mental Fatigue or Physical Fatigue and Scale of Stress

[0218] 28 to 30 healthy subjects were subjected to a 3-hour continuous simple calculation of the mental fatigue load, the Kraepelin test, or an aerobic treadmill exercise test with a pulse rate of 180 beats per minute as the physical fatigue load. The same blood collection as in Example 1 was carried out before and after, and the measurement of cytokines was carried out. Furthermore, the same measurement of cytokines was performed 24 hours after the start of the stress (21 hours after the end of the stress). The correlation coefficients of the blood concentrations of the respective cytokines were calculated, and the high correlation values ​​were marked with colors as the results.

[0219] From these results, it can be seen that the correlations of global cytokines changed before and after each of the above-mentioned tests and during the recovery period. By comparing the calculation as a me...

Embodiment 3

[0221] Example 3: Diagnostic scales and evaluation scales for mental disorders

[0222] Based on the international diagnostic standard DSM-IV diagnosis of 160 voluntary patients with mental disorders as much as possible questionnaire psychological test, using SDS, MAS, GHQ, STAI, SCID, CMI, subjective symptom survey (produced by the Industrial Health Research Institute), Social terror scale, crowd terror scale and independently developed living conditions questionnaire were used for investigation.

[0223] At the same time of the investigation, 1ml of blood was collected and put into the blood collection base cone containing anticoagulant, and the plasma was separated under the cooling condition of 4°C. Plasma was stored frozen or on ice, and the respective cytokines were measured simultaneously using a microbead protein array system (Bio-Plex Suspension Array System manufactured by Bio-Rad Laboratory independently modified by the present inventors). Cytokines were also mea...

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PUM

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Abstract

Disclosed are techniques capable of objectively and specifically evaluating various mental or physical conditions, of which evaluation was conventionally possible only by subjective symptom-dependent methods, such as stress and fatigue. Specifically disclosed are a stress or fatigue indicating agent including at least two factors selected from the group consisting of IL-1, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, IL-17, IL-18, Eotaxin, FGF basic, G-CSF, GM-CSF, IFN- , IFN-+-, IP-10, MCP-1, MIP-1+-, MIP-1, PDGF-BB, RANTES, TNF-+-, VEGF, CSF-2, TGF-, neurotrophin 5, MCP-3, -2-microglobulin, angiotensin II, CSF-3, CXC chemokine ligand 1, CXC chemokine ligand 5 and HGF; an agent for testing stress or fatigue including at least two molecules selected from the group consisting of molecules specifically recognizing the factors, respectively; a method of measuring stress or fatigue with the test agent; and an indicating agent for evaluating the intensity of mental conditions or disorders, including at least two factors selected from the factor group, wherein each of the factors is weighted.

Description

technical field [0001] The invention relates to a bioburden indicator and a method for determining bioburden. Specifically, the present invention relates to the technical field of objectively evaluating biological burdens such as stress and fatigue by means of molecular biology. Background technique [0002] Mental and / or physical stress affects host defense mechanisms including the nervous, endocrine and immune systems (see Non-Patent Documents 1 and 2). Various cytokines (for example, IL-1β, IL-6, TNF-α, etc.) are up-regulated by stress (see Non-Patent Documents 1 and 3). This suggests that cytokines may be involved in interference with host defense (see Non-Patent Document 4). However, the molecular mechanisms by which stress induces cytokines that can reduce host defense are still not well understood. [0003] Interleukin-18 (IL-18) is a cytokine discovered as an interferon-γ (IFN-γ) inducer (see Non-Patent Document 5 and Patent Document 1). IL-18 has various biologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N2800/301G01N33/6893G01N2800/302G01N2800/306G01N2800/304Y02A90/10
Inventor 关山敦生
Owner 关山敦生
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