Technology for solid phase synthesis of peptide by WANG strategy

Abstract

The invention relates to solid phase synthetic peptide technology by a WANG strategy, in particular to the synthetic mode taking six active oligopeptide as the target structure and adopting cross-matching of Boc-amino acid / strong acid-cleavage resin and Fmoc-amino acid / weak acid-cleavage resin, WANG (without acid-cleavage and nice gain) strategy for short, is adopted; finally the product with satisfactory yield and more lower cost is obtained.

Description

technical field [0001] This project involves some solid-phase synthesis methods of active peptides, which are especially suitable for economical and practical mass production of raw materials. Background technique [0002] For half a century, the solid-phase peptide synthesis method invented by Professor Merrifield has greatly promoted the development of medical science. So far, Boc / Bzl / strong acid hydrolysis resin method and Fmoc / tBut / weak acid hydrolysis resin method are the two most widely used protection strategies among many solid-phase peptide synthesis techniques. Their respective matching methods are as follows Figure 1 Show. [0003] Among the classic matching strategies mentioned above, Boc Chemical uses an acid gradient selective deprotection method. After each step of condensation, use a weak acid (such as TFA) to remove only the temporary protecting Boc group on the α-amino group; after all condensation is completed, use a strong acid (such as HF or TFMSA) to...

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Problems solved by technology

This is the inevitable result of the above two classic methods of peptide synthesis. The purity of the obtained crude product is poor, which increases the difficulty of further purification.

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