Method for preparing L-lactic acid by fermenting potato starch by Rhizopus oryzae
A technology of potato starch and rhizopus oryzae, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., which can solve the problems of high cost of immobilized cells, the production of L-lactic acid from potato starch, and the difficulty of pollution control, etc. problems, to achieve the effect of high yield of L-lactic acid, simple steps, and high biomass of fermentation products
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Embodiment 1
[0035] Taking fermentation in a 50L fermenter as an example, the process of producing L-lactic acid by solid-state fermentation of potato starch by Rhizopus oryzae is as follows:
[0036] 1. Preparation of Rhizopus oryzae Spores
[0037] The Rhizopus oryzae bacterial strain Rhizopus oryzae fungus is streak inoculated on the potato dextrose (PDA) slant medium, 32 ℃ of culture temperature are set on the first day, and relative culture humidity is 70%, is beneficial to mycelia growth; The second day sets culture temperature 34 ℃ ℃, the relative cultivation humidity is 30%, which is conducive to spore formation, inoculate the 3rd generation of activated strains by this method, and prepare to collect after the black dense spores completely cover the white hyphae.
[0038] 2. Preparation of Rhizopus oryzae spore emulsion suspension
[0039] Rinse the surface covered with mycelium with sterile water, inoculate in a 5L fermenter, and cultivate with seed medium until the concentration...
Embodiment 2
[0046] Taking fermentation in a 50L fermenter as an example, the process of producing L-lactic acid by solid-state fermentation of potato starch by Rhizopus oryzae is as follows:
[0047] 1. Preparation of Rhizopus oryzae Spores
[0048] The Rhizopus oryzae bacterial strain Rhizopus oryzae fungus is streak inoculated on the potato dextrose (PDA) slant medium, 32 ℃ of culture temperature are set on the first day, and relative culture humidity is 70%, is beneficial to mycelia growth; The second day sets culture temperature 34 ℃ ℃, the relative humidity of cultivation is 30%, which is conducive to spore formation. This method is used to inoculate 3-4 generations of activated strains. After the black dense spores completely cover the white hyphae, they can be ready to collect.
[0049] 2. Preparation of Rhizopus oryzae spore emulsion suspension
[0050] Rinse the surface covered with hyphae with sterile water, inoculate in a 5L fermenter, and cultivate to a concentration of 106 / m...
Embodiment 3
[0057] Taking fermentation in a 50L fermenter as an example, the process of producing L-lactic acid by solid-state fermentation of potato starch by Rhizopus oryzae is as follows:
[0058] 1. Preparation of Rhizopus oryzae Spores
[0059] The Rhizopus oryzae bacterial strain Rhizopus oryzae fungus is streak inoculated on the potato dextrose (PDA) slant medium, 32 ℃ of culture temperature are set on the first day, and relative culture humidity is 70%, is beneficial to mycelia growth; The second day sets culture temperature 34 ℃ ℃, the relative humidity of cultivation is 30%, which is conducive to spore formation. This method is used to inoculate 3-4 generations of activated strains. After the black dense spores completely cover the white hyphae, they can be ready to collect.
[0060] 2. Preparation of Rhizopus oryzae spore emulsion suspension
[0061] Rinse the surface covered with mycelium with sterile water, inoculate in a 5L fermenter, and cultivate with seed medium until th...
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