Method for detecting areca-nut yellow leaf disease phytoplasma pathogen and special reagent kit therefor

A phytoplasma and yellowing disease technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. problems, to achieve the effect of eliminating false positive contamination, simplifying the detection process, and eliminating the spread of seedlings
CN101475995BActive Publication Date: 2011-10-05ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Publication Date
2011-10-05

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Abstract

The present invention discloses a method for detecting betelnut yellow phytoplasma pathogen and its special kit. The kit includes a probe whose nucleotide sequence is the sequence 1 of the sequence table, and a primer pair composed of an DNA fragment whose nucleotide sequence is the sequence 2 of the sequence table and an DNA fragment whose nucleotide sequence is the sequence 3 of the sequence table. The method includes: extracting the DNA of the betelnut to be tested as a template, using the above kit to perform real-time fluorescence PCR, if the fluorescence signal is enhanced in the PCR process, the plant infects phytoplasma. Proved by experiment, the sensitivity of the inventive method detecting the betelnut phytoplasma infection is same with that of the traditional nested PCR electrophoresis detection, while the invention has simple operation and closed tube operation, processes automation control in the entire testing, eliminates the PCR false-positive contamination opportunities, does not need post-PCR handling, is suitable for bulk samples, and has simple, rapid, and accurate features.
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Description

technical field

[0001] The invention relates to a method for detecting the phytoplasma pathogen of betel nut yellowing disease and a special kit thereof, which are suitable for use in quarantine, agricultural production, plant protection and other departments. Background technique

[0002] Betel nut (Areca cathecu L.) is one of the important southern medicinal resources in my country. According to statistics from the Hainan Provincial Department of Agriculture, by the end of 2006, the area of ​​betel nut planted in Hainan Province reached 53,100 hectares, the harvested area was 23,300 hectares, and the total output was 74,800 tons. The output value is more than 1.8 billion yuan.

[0003] Betel nut yellowing is a devastating disease that first occurred in central Kerala, India. By the 1960s, betel nut yellowing had generally occurred in Quilon, Kerala state, India. In 1981, betel nut yellowing disease was first discovered in the medicinal materials field (Tunchang County) of ...

Claims

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