Method for fixing organization blocks by methanal stationary liquid

Abstract

The invention relates to a method for fixing a tissue block by a formaldehyde fixative. The method comprises the following steps: (1) soaking the tissue block into the neutral or subalkaline formaldehyde fixative in a reaction vessel and stirring the tissue block; (2) using an on-line pH detector to continuously detect the pH value of the fixative, recording the processing time when the pH value of the fixative reaches a subalkaline alarm pH value, and then replacing the fixative; and (3) repeating step (2) until the pH value of the fixative is still more than or equal to 7.0 when the fixative is treated for the time as the last processing time, namely reaching the reaction end point of the tissue block processing. The method is a method for fixing the tissue block by the formaldehyde fixative, which meets the scientific and technological principle and has source basis. The method can be used for commodity type automatic pathologic tissue processing equipment and realize the intelligent and full automatic working mode in the process of fixing the tissue block by the neutral (or subalkaline) formaldehyde fixative .

Description

technical field [0001] The invention belongs to the technical field of medical histopathology, in particular to a method for fixing tissue blocks with formaldehyde fixative. Background technique [0002] In the traditional medical histopathology technique, 4% formaldehyde fixative is used to fix pathological specimens to obtain tissue blocks. Generally, the formaldehyde fixative with a weight-to-volume ratio of 1:10 to 20 (g / ml) is used to soak the tissue blocks multiple times. It takes no less than 4 hours to fix and harden the tissue block, which belongs to the conventional step-by-step processing method of artificially setting the action process following the tradition. There is no traceable monitoring method for the tissue block fixation process; The differences in the facility conditions of the science and technology room, the professional technical ability of the personnel, and the difference in human work habits, the tissue block fixation process is not very consisten...

AI Technical Summary

Problems solved by technology

[0002] In the traditional medical histopathology technique, 4% formaldehyde fixative is used to fix pathological specimens to obtain tissue blocks. Generally, the tissue blocks are soaked in formaldehyde fixative with a weight-to-volume ratio of 1:10-20 (g / ml) for several times. It takes no less than 4 hours to fix and harden the tissue block, which belongs to the conventional step-by-step processing method of artificially setting the action process of the traditional follow-up. There is no monitoring method with traceability basis for the tissue block fixation process; The differences in the facility conditions of the science and technology room, the professional technical ability of the personnel, and the difference in human work habits, the tissue block fixation process is not very consistent, often resulting in poor tissue fixation and different effects, resulting in differences in the morphology and structure of the sliced ​​​​tissue cells under the microscope. Main technical factors affecting histopathological observation and diagnosis, which may easily lead to deviations in histopathological observation and diagnosis