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Plant promoting bacteria, microbial preparation containing the same and preparation thereof

A technology of plant growth-promoting bacteria and biological preparations, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting the reproduction opportunities of other pathogenic microorganisms, and achieve the goal of increasing yield, promoting plant growth, and improving fertilizer efficiency. Effect

Inactive Publication Date: 2009-07-15
黄晓东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3. Biological control of harmful microorganisms. Due to the inoculation of microbial fertilizers at the roots of crops, microorganisms grow and reproduce in large numbers at the roots of crops. As the dominant bacteria in the rhizosphere of crops, the reproduction opportunities of other pathogenic microorganisms are limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Example 1 The composition of the biological preparation of the present invention:

[0143] Composite carrier material: 25% peat (organic matter), 22% humic acid, 22% decomposed organic matter, 12% complex replacement agent (enzymolysis (digestion) protein 8% + calcium sulfate 4%), 0.05% methyl cellulose, the rest of the granulating agent;

[0144] Bacteria solution: The bacteria solution containing UW4, the concentration is 0.05ml, reaching 200 million bacteria.

[0145] Preparation:

[0146] First carry out the above-mentioned industrial fermentation of the UW4 strain to make a fermentation liquid;

[0147] Then 25% peat (organic matter) of the composite carrier material, 22% humic acid, 22% decomposed organic matter, 12% complex replacement agent, and 0.05% methyl cellulose are mixed;

[0148] Add 0.05ml of fermented bacteria liquid per gram of composite carrier material to reach 200 million bacteria per gram of composite carrier material; then add granulating agen...

Embodiment 2

[0169] Example 2 Composition of the biological preparation of the present invention

[0170] Composite carrier material: 27% peat (organic matter), 20% humic acid, 20% humus, 13% complex replacement agent (including enzymatic (digested) protein 10% + calcium sulfate 3%), 0.05% The methyl cellulose and the rest of the granulating agent; the bacteria liquid contains the UW4 strain solution, and the concentration is 0.01ml to reach 200 million bacteria.

[0171] Preparation method: Referring to Example 1, the soil for preparing the fermentation broth is different from the plant rhizosphere soil in Example 1, and the latitude and longitude of the geographical location of the soil in Example 1 are very different, and belong to plant rhizosphere soil in different regions.

[0172] According to the method identification and verification of the 16s rRNA gene program, the strain contained in the fermentation broth obtained above and the biological preparation is UW4.

[0173] The UW4 ...

Embodiment 3

[0174] Example 3 Composition of the biological agent of the present invention

[0175] Composite carrier material: 22% peat (organic matter), 22% humic acid, 22% humus, 16% complex replacement agent (including enzymatic (digested) protein 11% + calcium sulfate 5%), 0.05% The methyl cellulose and the rest of the granulating agent; the bacteria solution contains the UW4 strain solution, and the concentration is 0.1ml to reach 200 million bacteria.

[0176] Preparation method: Referring to Example 1, the soil for preparing the fermented bacterial liquid is different from the plant rhizosphere soil in Example 1, and the geographic location latitude and longitude of the soil in Example 1 is very different, and belongs to the plant rhizosphere soil in different regions.

[0177] According to the method identification and verification of the 16s rRNA gene program, the strain contained in the fermentation broth obtained above and the biological preparation is UW4.

[0178]Utilize the...

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Abstract

The invention provides a plant growth-promoting bacterium and a biological formulation containing the same, as well as a method for preparing the strain and the biological formulation; the biological formulation contains a composite carrier material and bacteria liquid, the composite carrier material contains organic matters, humic acids, decomposed organic matters, a complexing replacement agent and an embedding material; the bacteria liquid is a solution containing the plant growth-promoting bacterium UW4, the composite carrier material is added with the bacteria liquid such that the number of the plant growth-promoting bacteria runs up to at least 0.1 billion / per gram of the composite carrier material so as to obtain the biological formulation; the biological formulation is used for agricultural production, which can promote the growth of the plants, rise the yield, improve fertilizer effect and enhance the adversity resistance of the plants.

Description

technical field [0001] The invention relates to a microbial fertilizer, in particular to a plant growth-promoting bacterium and a microbial preparation, especially a biological preparation containing multifunctional plant growth-promoting bacterium components. Background technique [0002] Microbial fertilizer refers to a specific preparation containing living microorganisms, which can obtain specific fertilizer effects when applied in agricultural production. Microbial fertilizers can be divided into two categories. One is to increase the supply of plant nutrients through the life activities of microorganisms contained in it, improve the nutritional status of plants, and then increase production. The representative variety is bacterial fertilizer; the other is generalized fertilizer. Microbial fertilizers, although their products also increase crop production through the microbial life activities contained in them, but it is not limited to improving the supply level of plan...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/40
Inventor 黄晓东
Owner 黄晓东
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