Method for refining vegetable seed crude oil using immobilized phospholipase A1

A technology of immobilized phospholipase and immobilized enzyme, which is applied in the fields of edible oil/fat, application, food science, etc., can solve the problems of high cost of phospholipase, enzyme inactivation, difficult control of enzyme reaction conditions, etc., and achieve reduced sensitivity , high retention activity, and easy operating conditions

Inactive Publication Date: 2009-07-22
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is in order to solve the problem that enzyme reaction condition is difficult to control in actual production and cause enzy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0006] Specific embodiment one: use immobilized phospholipase A in this embodiment 1 The refining of rapeseed crude oil is achieved through the following steps: 1. Dissolve 1.0-15g of sodium alginate in 50-500g of distilled water for later use, and prepare 5-50mL of phospholipase A 1 Add it to 50-500mL HAc-NaAc or citric acid buffer solution with a pH value of 5 to obtain the enzyme solution for later use; 2. Mix the sodium alginate solution and the enzyme solution at a volume ratio of 0.5-3:1, Obtain a mixed solution; 3. Dissolve 1.0-15g of chitosan in 50-500g of 2-10% acetic acid solution, and then add it to prepare 100-1000mL of 0.05-0.2mol / L CaCl 2 solution, the mixture was added dropwise to CaCl 2 In the mixed solution, round particles are formed, and the particles are left to stand for 40-60 minutes and then dried at 30-50°C to obtain granular immobilized enzymes for use; 4. Take the rapeseed crude oil and go through the hydration degumming method first Remove the hydr...

specific Embodiment approach 2

[0008] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, 2-12 g of sodium alginate is dissolved in 80-400 g of distilled water for later use. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0009] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step one, 10-40mL phospholipase A 1 Add to 100-400mL HAc-NaAc or citric acid buffer solution with pH value of 5. Other steps are the same as in the first embodiment.

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Abstract

The invention provides a method for refining rape seed crude oil by immobilized phosphatidase A1, and relates to the method for refining the rape seed crude oil. The problems of inactivation of enzyme caused by an uneasily controlled enzyme reaction condition during production and high cost of phosphatidase in enzyme process degumming can be solved by selecting the immobilized phosphatidase A1 for degumming. The method for refining the rape seed crude oil by the immobilized phosphatidase A1 is realized by the following steps: 1, preparing a reaction solution; 2, preparing a mixed solution; 3, preparing granulated immobilized enzyme; 4, pretreating the rape seed crude oil; 5, adding the immobilized enzyme into the oil; and 6, stirring and centrifugating to realize the refined rape seed crude oil by the immobilized phosphatidase A1. The rape seed crude oil is subjected to the degumming treatment by the method to improve degumming efficiency. Compared with the prior method, the method improves the degumming efficiency by 5 to 30 percent and the economic benefit by 1 to 3 percent.

Description

Technical field: [0001] The invention relates to a method for refining rapeseed oil. Background technique: [0002] Degumming is an important link in the refining process of rapeseed oil. It is a necessary condition to ensure the smooth progress of physical refining and is crucial to improving the refining rate and oil quality. Gum removal is not complete, which not only affects the deep processing of oil products and the stability of oil products, but also affects the cooking and edible properties of oil products. The main purpose of degumming is to remove phospholipids in oil, and phospholipids are divided into hydratable phospholipids and non-hydratable phospholipids. In the degumming process, hydratable phospholipids can be removed by simple hydration degumming methods. Non-hydratable phospholipids have obvious Hydrophobic, it is difficult to combine with water in hydration degumming, and it is difficult to remove. Due to the influence of climate in the maturity stage ...

Claims

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Application Information

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IPC IPC(8): A23D9/04
Inventor 于殿宇王世让王瑾罗淑年杨帆李默馨曹万新魏冰
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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