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Lung cancer diagnostic assay

A lung cancer, molecular technology, applied in the direction of analytical materials, instruments, biochemical equipment and methods, etc., can solve the problems of unsuitable screening programs, expensive PET scans, etc.

Inactive Publication Date: 2009-07-22
UNIV OF KENTUCKY RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] PET scans are another diagnostic option, but PET scans are expensive and generally unsuitable for screening programs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Example 1-NSCLC diagnostic analysis using T7 clone

[0152] In this example, the identification of markers for the diagnosis of advanced (II, III, and IV) NSCLC was performed. Two T7 phage NSCLC libraries were screened using NSCLC patient and normal human plasma bio-panning to enrich the population of immunogenic clones expressing polypeptides recognized by circulating antibodies in NSCLC patients.

[0153] A T7 phage NSCLC cDNA library (Novagen, Madison, WI) was purchased, and Novagen OrientExpress cDNA synthesis and cloning system was used, and the other library was constructed from the adenocarcinoma cell line NCI-1650. A biological panning library of mixed plasma from 5 NSCLC patients (stage 2-4; diagnosis confirmed by histology) and normal healthy donors was used to enrich the phage-expressed protein group recognized by tumor-associated antibodies. In short, the phage-displayed library is incubated with protein G Sepharose beads coated with antibodies from mixed normal...

Embodiment 2

[0184] Example 2-Using T7 clone to detect early lung cancer

[0185] In this example, the ability of the present invention to identify markers that can distinguish between lung cancer stage I and occult disease from risk-matched control samples according to the present invention was investigated.

[0186] Human subject

[0187] After notification and permission, plasma samples were obtained from individuals with histologically confirmed NSCLC at the University of Kentucky and Veterans Administration Medical Center. A cancer-free control was randomly selected from 1520 subjects who participated in the Mayo Clinic Lung Screening Trial. In short, individuals who have a smoking history of at least 20 years, are between 50-75, and have no other malignancies within the five years before entering the study are eligible for CT screening tests. In addition to cancer-free samples from the Mayo lung screening test, 6 NSCLCI stage samples and 40 pre-diagnostic samples are also available for...

Embodiment 3

[0209] Example 3-Using the same method to identify lung cancer-specific random peptide markers and develop NSCLC diagnostic scores Analyze

[0210] Lung cancer-specific markers are also obtained by using phage-displayed random peptides. Such libraries are commercially available or can be constructed as known in the art. M13 was selected as the carrier.

[0211] Mark identification

[0212] Commercially available applications include 2×10 fused to secondary coat protein 9 A random peptide M13 phage display peptide library (Ph.D. TM -C7C, NEB). Each phage clone expresses a unique 7 amino acid peptide in a ring structure on the surface of the phage. The ring structure is bound by a single flanking disulfide bond formed in the periplasm of the bacteria.

[0213] The library was subjected to two rounds of "biopanning" with plasma from lung cancer patients and controls as described above. The biopan library is then amplified for the isolation of individual phages. A robot for automa...

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Abstract

A diagnostic assay for determining presence of lung cancer in a patient depends, in part, on ascertaining the presence of an antibody associated with lung cancer. The assay predicted lung cancer prior to evidence of radiographically detectable cancer tissue.

Description

[0001] Government rights [0002] Some of the studies disclosed here are supported by the National Institutes of Health (R01, CA10032-01), the Veteran's Administration Merit Review Program and the Kentucky Lung Cancer Research Management Center (KentuckyLung Cancer Research). Administration) to provide funding. Background technique [0003] In the United States and many other countries, lung cancer is the leading cause of cancer death not only for men but also for women. In the past five years, the number of deaths caused by this disease has been on the rise every year, and the United States alone has reached about 164,000 cases, most of which are due to non-small cell carcinoma (NSCLC). This exceeds the combined mortality of breast, prostate and colorectal cancer. [0004] Many experts believe that early detection of lung cancer is the key to improving survival rates. Studies have shown that when the disease is detected in the early, localized stage and can be removed surgically, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/537G01N33/538G01N33/564G01N33/566
CPCG01N33/57423C12Q1/6886G01N33/564G01N33/574G01N33/537
Inventor 纳达·H·卡塔爱德华·A·希尔施科维奇钟丽阿诺德·J·斯托姆博格
Owner UNIV OF KENTUCKY RES FOUND
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