Method for producing induced multipotential stem cell

A technology of pluripotent stem cells and cells, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., can solve problems such as low efficiency, affecting application and promotion, and achieve high efficiency
CN101550428AInactive Publication Date: 2009-10-07SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Publication Date
2009-10-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for producing induced multipotential stem cell, comprising steps of: A) constructing viral vectors carrying transcription factors, wherein the transcription factors are selected from: Oct4, Sox2, c-Myc, Klf4, Lin28 and Nanog; B) respectively transfecting viral vectors obtained in step A), and obtaining virus liquid containing the transcription factors; and C) infecting cells in combination manner by the virus liquid containing the transcription factors obtained from step B)selecting clones with human embryo like stem cell for subculturing, and obtaining induced multipotential stem cell by screening cell clone according to human embryo stem cell characteristic. The method of the invention for preparing human embryo stem cell has high efficiency, can avoid rejection reaction, differentiates to be different histiocytes in specific condition, and has an extensive application future.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a method for preparing induced pluripotent stem cells (Induced pluripotent stem cells, referred to as iPS cells). Background technique

[0002] Human embryonic stem cells have the potential to differentiate into various types of human cells, and provide the possibility of application for regenerative medicine (Thomson JA., Itskovitz-Eldor J., Shapiro SS., et al.Science.Nov 6 1998; 282( 5391): 1145-1147). However, immune rejection has a great influence on the transplantation therapy of human embryonic stem cells. In order to be able to cultivate patient-specific pluripotent stem cells to eliminate the impact of immune rejection, people have tried many methods, such as nuclear transfer of adult cells (also known as therapeutic cloning), fusion of adult cells and human embryonic stem cells, etc. , but were not successful. The possibility of obtaining patient-specific induce...

Claims

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