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Radiation pneumonitis related genes ATM and P53 as well as polymorphic locus and application thereof

A gene and locus technology, applied in the gene ATM and P53 and their polymorphic loci and application fields, can solve problems such as diseases and phenotype changes

Inactive Publication Date: 2009-10-07
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the non-coding region SNP, if the SNP exists in the regulatory region of the gene and causes changes in gene expression levels or changes in mRNA stability and translation processes, it may lead to changes in phenotypes and even the occurrence of diseases

Method used

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  • Radiation pneumonitis related genes ATM and P53 as well as polymorphic locus and application thereof
  • Radiation pneumonitis related genes ATM and P53 as well as polymorphic locus and application thereof
  • Radiation pneumonitis related genes ATM and P53 as well as polymorphic locus and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: detection kit

[0027] 1) Extraction of DNA template

[0028] 2 ml of human peripheral blood was extracted by conventional methods, and genomic DNA was extracted by standard phenol-chloroform extraction method, and the DNA concentration was corrected to 50 ng / μl.

[0029] 2) PCR reaction

[0030] Prepare the PCR product that detects ATM and P53 gene single nucleotide polymorphic site, wherein, contain following two pairs of primers that can amplify -111G→A and P53 gene Arg72Pro single nucleotide polymorphic site:

[0031] Sense primer: 5'-CTTGGCGTTGCTTCTTCCTC-3' (SEQ ID NO: 16)

[0032] Antisense primer: 5'-CGCAGATCCCGACTCCTCT-3' (SEQ ID NO: 17)

[0033] and

[0034] Sense primer: 5'-TTGCCGTCCCAAGCAATGGAT-3' (SEQ ID NO: 19)

[0035] Antisense primer: 5'-TCTGGGAAGGGACAGAAGAT-3' (SEQ ID NO: 29)

[0036] The PCR reaction conditions were 95°C for 2 minutes, 35 cycles of 94°C for 30 seconds, 60°C for 30 seconds and 72°C for 30 seconds, and finally 72°C fo...

Embodiment 2

[0044] Example 2: Correlation between ATM and P53 Gene SNPs and Susceptibility to Radiation Pneumonia

[0045] Statistical method: use the Pearson chi-square test in SPSS 11.0 software to calculate the Hardy-Weinberg balance of ATM and P53 gene single nucleotide polymorphisms, and calculate the distribution of allele frequency and genotype frequency in the diseased group and the non-affected group For differences, the statistical significance level was set at P<0.05. Logistic regression analysis was used to calculate the OR value and its 95% confidence interval (CI) of the risk of radiation pneumonitis.

[0046] Blood sample collection: Peripheral blood samples of patients with lung cancer who underwent radiotherapy were collected from the Cancer Hospital of the Chinese Academy of Medical Sciences. The basic information of the patients is shown in Table 5. All subjects signed an informed consent form, and this study was also approved by the institutional ethics committee.

...

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Abstract

The invention relates to radiation pneumonitis related genes ATM and P53 as well as polymorphic loci and an application thereof. An ATM gene promoter region-111G, an A mononucleotide polymorphic locus rs189037 and a functional single nucleotide polymorphism of a P53 gene coding region Arg72Pro during the single or cooperative action are related with the occurrence of radiation pneumonitis after radiation therapy. After the polymorphic loci of the ATM genes and the P53 genes of a pulmonitis patient are carried out gene typing, an individual easy to be infected with the radiation pneumonitis after radiotherapy can be identified, thus the invention can be applied to prevent the occurrence of the radiation pneumonitis.

Description

technical field [0001] The invention relates to the gene field of biotechnology, in particular, the invention relates to a gene ATM and P53 related to radiation pneumonitis and its polymorphic site and application. Background technique [0002] In my country, lung cancer is a very common malignant tumor. Radiation therapy is an effective lung cancer treatment that has been widely used. Graphical guidance technology represented by three-dimensional conformal radiotherapy and intensity-modulated radiotherapy has greatly improved the control effect of lung cancer while reducing its toxicity. Nevertheless, normal lung tissue adjacent to or surrounding the tumor in the radiation field is damaged by radiation, leading to radiation pneumonitis. In patients with radiation pneumonitis, mild cases are asymptomatic, and the inflammation can dissipate spontaneously; severe cases have extensive fibrosis in the lungs, leading to respiratory function damage and even respiratory failure. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/12A61P11/00
Inventor 林东昕王绿化杨明张莉谭文
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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