Use of butin and derivatives thereof for preparing drugs having inhibitive effect on immune system
A technology for the immune system and its derivatives, which is applied in the field of application of buteanin and its derivatives in the preparation of drugs with the effect of suppressing the immune system, and can solve the problems of unclear active ingredients of Vernonia anthelmintic
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experiment example 1
[0020] In Vitro Pharmacodynamics Study of Experimental Example 1 Butacein
[0021] Due to the impairment of pigment cells and their functions in skin lesions of patients with vitiligo, we studied the effect of butycin on human melanocytes cultured in vitro.
[0022] 1.1. Experimental scheme
[0023] 1.1.1 Screening model: normal human melanocytes;
[0024] 1.1.2. Source: redundant foreskin from circumcision of children, without infection.
[0025] 1.1.3. Culture conditions: take normal children’s foreskin and trypsinize it to obtain melanocytes, add bFGF, CT, TPA, FCS, double antibody and other factors to DMEM medium, 5% CO 2 , cultured at 37°C for 7 days and subcultured, the subcultured cells were cultured under the above conditions for 48 hours, and then different concentrations of drugs and controls were added to determine the relevant analysis indicators.
[0026] 1.1.4. Determination method: MTT method for cell proliferation; Dopa staining method for tyrosinase activit...
experiment example 2
[0046] Experimental example 2. In vivo pharmacodynamics study of butycin
[0047] The effect of butycin on the proportion of melanocytes in the basal layer of brown guinea pigs was observed by intragastric administration.
[0048] 2.1. Experimental scheme
[0049] Experimental animals: British short-haired brown guinea pigs, provided by Xinjiang Experimental Animal Center.
[0050] Test drug settings:
[0051] Butein:
[0052] High concentration group: 8.74mg / kg (calculated on the basis of 1 / 25 of the maximum tolerated dose)
[0053] Medium concentration group: 4.37mg / kg (calculated on the basis of 1 / 50 of the maximum tolerated dose)
[0054] Low concentration group: 2.19mg / kg (calculated on the basis of 1 / 100 of the maximum tolerated dose)
[0055] Positive control group: 8-MOP (8-methoxy psoralen, China National Institute of Pharmaceutical and Biological Products) Negative control group: 2.5% hydroquinone blank control group; normal saline
[0056] 2.2. Establishment o...
experiment example 3
[0060] Experimental Example 3. Effects of Butycin on the Immune System of Mice
[0061] 3.1. Experimental scheme
[0062] 3.1.1. Experimental model: Culture Kunming newborn mouse splenocytes.
[0063] 3.1.2. Animal source: provided by Xinjiang Experimental Animal Center.
[0064] 3.1.3. Culture conditions: spleen cells were obtained from the spleen of Kunming newborn mice after trypsinization.
[0065] Add FCS, double antibody and other factors to RPMI1640 medium, 5% CO 2 After culturing at 37°C for 48 hours, different concentrations of drugs and controls were added to determine the relevant analysis indicators.
[0066] 3.2. Results
[0067] Since the current etiological research supports that the pathogenesis of vitiligo is related to the immune system, it is found that there are specific antibodies in the body of patients with vitiligo. The results of research on the effect of butycin on the immune system of mice showed that butycin has inhibitory effects on cellular i...
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