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Method for preparing Ulva pertusa Kjellm extract for marine antifoulant

A technology of marine antifouling agents and extracts, which can be used in botanical equipment and methods, chemicals for biological control, paints containing biocides, etc., can solve problems such as damaging the environment, and achieve strong antifouling effects and raw materials Easy access, strong growth inhibitory effect

Active Publication Date: 2013-06-19
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the prior art, aiming at the problems that marine antifouling agents such as organotin and DDT seriously damage the environment and urgently need environmental protection substitutes, it is designed to provide a preparation method using the extract of natural marine green alga Ulva ulvae The method of antifouling agent can well inhibit the adhesion of diatoms, mussels and other marine organisms, and achieve the purpose of non-toxic and environmentally friendly antifouling

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Preparation of a kind of Ulva pore extract A

[0014] Collect fresh Ulva pore from the intertidal zone, air-dry it naturally, and crush it into about 1cm with a grinder 2 Size, weigh 250g into a flask, add ethyl acetate until completely submerged Ulva pore, heat in a water bath at 50°C, soak for 48 hours, filter, filter residue remains in the flask, then add ethyl acetate until completely submerged, repeat extraction Three times, the solvent was removed from the filtrate by rotary evaporation to obtain a black paste, which was Ulva pore solvent extract A.

Embodiment 2

[0015] Embodiment 2: the preparation of a kind of Ulva pore extract B

[0016] Get 60g of 200 mesh silica gel and activate it in an oven at 110° for 2 hours, then use petroleum ether to wet-pack the column, get 0.5g of Ulva pore extract A prepared in Example 1, dissolve it in a small amount of ethyl acetate, and wet-pack the column. Samples were eluted with petroleum ether, ethyl acetate, and ethanol respectively, and the effluent was collected according to the color of the layers, and 6 isolated products were collected accordingly. After the fifth isolated product was collected, it was evaporated by rotary evaporation to remove the solvent. After that, Ulva pore extract B was obtained.

Embodiment 3

[0017] Embodiment 3: the preparation of a kind of Ulva pore extract C

[0018] Get 50g of 300 mesh silica gel and activate it in an oven at 110° for 2 hours, then use petroleum ether to wet-pack the column, get 0.5g of Ulva pore extract B prepared in Example 2, dissolve it in a small amount of ethyl acetate, and wet-pack the column. Sample was eluted with a mixed solution of ethyl acetate and ethanol (mixing ratio: 1:20), and the effluent was collected according to the color of the layers, and 4 isolated products were collected accordingly. After the second isolated product was collected, it was spun After evaporating and removing the solvent, Ulva pore extract C is obtained.

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PUM

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Abstract

The invention relates to preparation of a biological antifoulant for preventing biofouling on the surface of an artificial material in the technical fields of marine environments and antifouling, in particular to a method for preparing an Ulva pertusa Kjellm extract for a marine antifoulant, which comprises the following steps: placing the fresh Ulva pertusa Kjellm which are dried in the air and crushed into a container, adding a solvent into the container till the fresh Ulva pertusa Kjellm are completely immersed in the solvent, heating the solution under reflux at a constant temperature of between 30 and 80 DEG C for 1 to 50 hours, and filtrating the solution to obtain a liquid solvent extractable matter; concentrating the liquid solvent extractable matter to remove the solvent to obtainan pasty extract A; and separating solvent extractable matter A by column chromatography, subjecting the separated solvent extractable matter A to gradient elution with sherwood oil, ethyl acetate and ethanol respectively to obtain mixed separated product B sequentially, subjecting the separated product B to separation by column chromatography, and eluting the product of the separation by columnchromatography with a mixed solution prepared from the ethyl acetate and the ethanol in weight ratio of from 1:20 to 20:1 to obtain a separated product C which is the Ulva pertusa Kjellm extract product. The Ulva pertusa Kjellm extract has the advantages of nontoxicity, environmental protection, readily available raw materials, simple preparation, high quality, high antifouling effect, and the like.

Description

Technical field: [0001] The invention relates to the preparation of a biological antifouling agent for preventing and removing biological fouling on the surface of artificial materials in a marine environment, in particular to a preparation method of an Ulva ulva extract for a marine antifouling agent, and belongs to the technical field of marine biological antifouling. Background technique: [0002] Marine biofouling refers to the adverse effects of biofouling on the surface of marine structures on sea-soaked materials. The organisms attached to the surface of structures are called marine fouling organisms, including barnacles, mussels, sea squirts, bryozoans, hydra, sponges, green algae, diatoms, etc. The surface of the above-mentioned structures includes ships, docks, docks, oil platforms, submarine cables, buoys, mines, power plant cooling pipes, fishing nets, etc. Marine biofouling causes serious harm, such as increased navigational resistance of ships, blockage of sea...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P13/00A01P17/00C09D5/16
Inventor 郑纪勇蔺存国段东霞
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP