Preparation method of multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement

A calcium phosphate bone cement and gelatin technology, applied in prosthesis, medical science, bone implants, etc., can solve the problem of not being able to meet the timing and quantitative release of various drugs

Inactive Publication Date: 2009-10-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned carriers are mostly used to load one drug, which cannot meet the timed and quantitative release of multiple drugs required for bone repair treatment.

Method used

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  • Preparation method of multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement
  • Preparation method of multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement
  • Preparation method of multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Preparation of gelatin microspheres

[0020]Dissolve 3g of gelatin in 30mL of deionized water at a temperature of 40°C to prepare a gelatin solution with a concentration of 0.10g / mL; then add the gelatin solution into 125mL of vegetable oil with continuous stirring (stirring speed 700rpm) through a porous nozzle. Keep at 15°C, stir for 10 minutes to form an emulsion, quickly change to an ice bath while keeping the stirring speed constant, keep the system temperature below 4°C, continue stirring for 15 minutes, slowly add 5mL of glutaraldehyde with a concentration of 25% dropwise Solution, continue to stir for 0.5h, after filtering, soak the microspheres in the glycine solution with a concentration of 1mol / L for 30min, remove unreacted glutaraldehyde, and wash the prepared microspheres with acetone and ethanol for 3 times, and dry naturally The gelatin microspheres (such as figure 1 shown), the microspheres were sieved and classified to obtain gelatin microspher...

Embodiment 2

[0026] Step 1: Preparation of gelatin microspheres

[0027] Dissolve 9g of gelatin in 30mL of deionized water at a temperature of 40°C to prepare a gelatin solution with a concentration of 0.3g / mL; then add the gelatin solution dropwise into 125mL of vegetable oil with continuous stirring (stirring speed 500rpm) with a syringe, and the oil Keep the temperature at 38°C, stir for 30 minutes to form an emulsion, quickly change to ice bath under the condition of keeping the stirring speed constant, keep the system temperature below 4°C, continue stirring for 30 minutes, slowly add 10mL of 25% pentadiol dropwise Aldehyde solution, continue to stir for 0.5h, after filtration, soak the microspheres in 1mol / L glycine solution for 30min, remove unreacted glutaraldehyde, wash the prepared microspheres with acetone and ethanol three times in sequence, and dry naturally to obtain The gelatin microspheres with a particle size ranging from 100 to 250 μm are sieved and classified to obtain g...

Embodiment 3

[0033] Step 1: Preparation of gelatin microspheres

[0034] Dissolve 9g of gelatin in 30mL of deionized water at a temperature of 40°C to prepare a gelatin solution with a concentration of 0.3g / mL; then add the gelatin solution dropwise with a syringe to 125mL of vegetable oil with continuous stirring (stirring speed 300rpm). Keep the temperature at 50°C, stir for 30 minutes to form an emulsion, quickly change to ice bath under the condition of keeping the stirring speed constant, keep the system temperature below 4°C, continue stirring for 60 minutes, slowly add 10mL of 50% pentadiol dropwise aldehyde solution, continue to stir for 1 h, after filtration, soak the microspheres in 1mol / L glycine solution for 30 min to remove unreacted glutaraldehyde, wash the prepared microspheres with acetone and ethanol three times in sequence, and dry naturally to obtain particles Gelatin microspheres with diameters ranging from 75 to 150 μm were sieved and classified to obtain gelatin micro...

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Abstract

A preparation method of a multistage drug release carrier compounded by gelatin microspheres and calcium phosphate cement takes plant oil as continuous phase, disperses the aqueous solution of gelatin in the plant oil to form emulsion and obtains the gelatin microspheres of different grain diameters by controlling the temperature and stirring time of the plant oil; corresponding substances is weighed according to the mass rate (5-20): (95-80) of the gelatin microspheres to the calcium phosphate cement powder, compound powders are obtained after uniform mixing and formulates compound powder sizing agent is prepared by blending NaH2PO4 with the mass percent concentration being 0.8 percent with the compound powder under room temperature for 60-90s; and the blended sizing agent is filled into a mold to be solidified for 1-2h under the conditions of constant temperature of 37 DEG C and 100 percent humidity and finally the compound carrier is obtained. The gelatin microspheres and the bone cement are compounded to construct the drug release compound carrier and encapsulated drug types are designed according to the difference of degradation rates of the gelatin microspheres, so as to realize the timed and quantitative release of different drugs, and simultaneously, as a bone repair support, the drug release carrier can finally substitute a calcium phosphate cement carrier support.

Description

technical field [0001] The invention relates to a preparation method of a gelatin microsphere / calcium phosphate bone cement composite multi-stage drug release carrier, belonging to the technical field of biomedical material preparation. Background technique [0002] With the frequent occurrence of traffic accidents and the aging of the population, more and more patients have bone defects caused by fractures, osteomas or osteoporosis. Therefore, research on bone repair therapy has attracted more and more attention. Traditional bone repair treatments are prone to postoperative complications, and these reactions occur and increase sequentially in the days, weeks, and months after surgery. Therefore, different drugs need to be used for treatment at different periods after surgery. If a variety of drugs can be loaded at one time and released sequentially at regular intervals, it will greatly reduce the suffering of patients. At present, oral administration or injection is often ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42A61L27/24A61L27/12A61F2/28
Inventor 蔡舒卢姗姗周维翟羽佳许国华
Owner TIANJIN UNIV
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