A method for auxiliary screening of powdery mildew resistant wheat and its special primers

A technology for resistance to powdery mildew and wheat, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and applications, and can solve problems such as hidden dangers in production, strong reproductive ability, and loss of resistance of major disease resistance genes.
CN101570790BInactive Publication Date: 2011-12-21INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Publication Date
2011-12-21
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a powdery mildew resistant wheat auxiliary screening method and a special primer thereof. The powdery mildew resistant wheat auxiliary screening primer pairs provided by the invention are at least one pair of the primer pairs of Xbarc79-1 and Xwmc288-1; the deoxyribonucleotide sequence of the primer pairs Xbarc79-1 is shown as sequence 1 and sequence 2 in a sequence table; the deoxyribonucleotide sequence of the primer pair Xwmc288-1 is shown as sequence 3 and sequence 4 in the sequence table. The invention also provides molecular markers Xbarc79 and Xwmc288 of powdery mildew resistant gene, the deoxyribonucleotide sequence of the molecular marker Xbarc79 is shown as sequence 5 in the sequence table, and the deoxyribonucleotide sequence of the molecular marker Xwmc288 is shown as sequence 6 in the sequence table. The primer pairs and the molecular marker of the invention can play an important role in wheat breeding for disease resistance.
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Description

technical field

[0001] The invention relates to a method for assisting in screening wheat resistant to powdery mildew and special primers thereof. Background technique

[0002] Wheat powdery mildew is a fungal disease caused by wheat powdery mildew (Blumeria graminis f.sp.tritici), which mainly occurred in mild climate, humid and rainy areas in the past. With the popularization of dwarf and semi-dwarf varieties of wheat and the continuous improvement of water and fertilizer conditions, the production level of wheat has been greatly improved. The high density caused by large sowing volume, unreasonable cultivation measures such as partial application of nitrogen fertilizer, excessive irrigation, etc., artificially The induction condition of powdery mildew is provided, and the harm of powdery mildew is aggravated day by day. Before the 1970s, wheat powdery mildew was mainly prevalent in the humid and rainy southwest regions such as Yunnan and Guizhou and the coastal areas of ...

Claims

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