Chicken Marek's virus antibody and preparation method thereof

A technology of Marek's virus and Marek's disease, which is applied in the field of biological preparations and its preparation, can solve the problems of heavy purification workload, high consumption of chloroform, and high cost of egg yolk antibody preparation, so as to reduce workload, make preparation safe, and reduce pathogens pollution effect

Inactive Publication Date: 2009-11-11
张训海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two methods for extracting egg yolk antibodies all have the following problems: since the egg yolk liquid or egg yolk supernatant is directly degreased and purified by chloroform first, and then ultrafiltration is performed, the consumption of chloroform is large, and the workload of purification is large. Antibody preparation costs are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method for chicken Marek's virus antibody, comprising the following steps:

[0027] (1) Select newborn SPF healthy chicks, inoculate Marek's disease (MD) virus (MDV) vaccine strain as basic immunity, and described vaccine strain is CVI988 strain (commercially available) of serotype I and carries out immunization according to instruction manual ;

[0028] 30 days after immunization, the MDV antigen adjuvant vaccine was subcutaneously injected with a dose of 0.5ml / feather to boost the immunization. The preparation method of the adjuvant vaccine was: the extracted and purified MDV A antigen was prepared into a water phase according to a conventional method and mixed with oil phase for emulsification preparation, requiring Qiong Kuo titer of 1:8 or more;

[0029] On the 60th day after the booster immunization, the MDV antigen adjuvant vaccine was injected intramuscularly at a dose of 1.0ml / feather for booster immunization, and the MDV antigen adjuvant vaccine...

Embodiment 2

[0035] A preparation method for chicken Marek's virus antibody, comprising the following steps:

[0036] (1) Select newborn SPF healthy chicks, inoculate Marek's disease (MD) virus (MDV) vaccine strain as basic immunity, and described vaccine strain is CVI988 strain (commercially available) of serotype I and carries out immunization according to instruction manual ;

[0037] After 45 days of immunization, the blood virus after the rejuvenation of the BJ-1 strain of pathogenic Marek's virus was diluted 100 times with the special diluent for MD vaccine, and then boosted by subcutaneous injection of 0.2ml / feather;

[0038] 60 days after the booster immunization, the MDV antigen adjuvant vaccine was injected intramuscularly with a dose of 1.5ml / feather for booster immunization, and the same booster immunization was carried out with the MDV antigen adjuvant vaccine every 2 months thereafter. The adjuvant vaccine The preparation method is as follows: the extracted and purified MDV A ...

Embodiment 3

[0044] A preparation method for chicken Marek's virus antibody, comprising the following steps:

[0045] (1) immunize newborn SPF healthy chicks with Marek's disease (MD) virus (MDV) serotype III turkey herpes virus Fc-126 strain (commercially available) according to the instructions, and then immunize them with Marek's 15 days after immunization. The CVI988 strain (commercially available) of the serotype I of the disease (MD) virus (MDV) vaccine strain carries out secondary immunization as basic immunization according to instructions;

[0046] 15 days after the basic immunization, the MDV antigen adjuvant vaccine was injected intramuscularly at a dose of 0.5ml / feather for booster immunization. The preparation method of the adjuvant vaccine was: after the extracted and purified MDV A antigen was made into an aqueous phase according to a conventional method For emulsification preparation with the oil phase, the titer of Qiong Kuo is required to be above 1:16;

[0047] 60 days ...

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Abstract

The invention discloses a chicken Marek's virus antibody and a preparation method thereof. The preparation method comprises the following steps: adopting at least one of Marek's disease (MD) virus (MDV) vaccine strain, expression vector containing an objective antibody, pathogenic Marek's virus and MDV antigen adjuvant vaccine to inoculate a chicken according to a certain inoculation program, monitoring the objective antibody potency generated by the inoculated laying chicken, and beginning collecting eggs produced by the inoculated laying chicken when the prescribed lowest objective antibodypotency is achieved; and separating egg yolks in the collected eggs, preparing egg yolk supernatant by adopting an acid water method, ultra-filtering the egg yolk supernatant to obtain concentrated solution, and carrying out degreasing and purification treatment for the concentrated solution by adopting chloroform to obtain the objective antibody. Therefore, on the basis of not influencing the specificity and sensibility of the antibody, the preparation of the antibody is safer, simpler, more convenient and feasible, and is convenient for industrialization; and the prepared antibody has high yield and low cost, and can be used for immune monitoring and plague prevention and control.

Description

technical field [0001] The invention relates to a biological preparation and a preparation method thereof, in particular to a chicken Marek's virus antibody and a preparation method thereof. Background technique [0002] Marek's disease (MD) is an immunosuppressive and tumorigenic infectious disease caused by Marek's herpes virus (MDV). It has a long incubation period and strong concealment, and is one of the main infectious diseases of chickens. Because the disease seriously damages the chicken immune system, except that it can cause a cumulative fatality rate of up to more than 70%, it can also often cause the immune failure or secondary infection of other chicken disease vaccines and cause heavy losses. MD vaccine immunization can only prevent or reduce the incidence of MD in infected chickens, but cannot block the invasion and spread of pathogenic MDV to chickens. Therefore, the diagnosis and monitoring of MDV infection is not only an important means of MD prevention an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02C07K1/14
Inventor 张训海王旋丁维民龚争孙格格张翠赵磊
Owner 张训海
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