Method for producing isomaltulose without purification step

A technology for isomaltulose and its production method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems affecting the quality of isomaltulose products and increasing production costs, etc.

Inactive Publication Date: 2009-11-11
DALIAN POLYTECHNIC UNIVERSITY
View PDF2 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conversion products of these sucrose isomerases also contain 2 to 7% by-products such as glucose and fructose, which seriously affect the quality of isomaltulose products, and must be separated and removed in the post-extraction proce...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing isomaltulose without purification step
  • Method for producing isomaltulose without purification step
  • Method for producing isomaltulose without purification step

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, conversion tank batch conversion production isomaltulose

[0029] Klebsiella LSI was inoculated from the reserved slant into sucrose medium (2% sucrose, 0.1% yeast powder, 0.1% peptone, 0.05% magnesium sulfate, 0.1% NaH 2 PO 4 , 0.1% KNO 3 , pH6.6) in the Erlenmeyer flask of activation culture 8 hours (30 ℃, 150 rev / min), transfer in the fermentor that fresh sucrose medium is housed by 5% inoculum amount, ventilate and cultivate after 12 hours, in fermentor Cells were sonicated under aseptic conditions to prepare a suspension of active cell fragments. In a 10-ton conversion tank, 5%, 10%, 15% and 20% sucrose solutions were respectively prepared, sterilized at 95°C for 20 minutes, and then cooled to 30°C. Transfer the above active cell debris suspension into a 10-ton transformation tank at a ratio of 1:10, keep it warm at 30°C, and pass sterile air to maintain the positive pressure state in the transformation tank. When the sucrose concentration is 5% a...

Embodiment 2

[0030] Embodiment 2, immobilized cell production isomaltulose

[0031] After every 5 ml of Klebsiella LSI bacterial culture solution was fully mixed with 100 ml of 2% sodium alginate solution, it was added dropwise to 0.65% (w / v) CaCl with a syringe 2 solution, and placed in the solution at 4°C for 4 hours to strengthen the immobilized particles. The immobilized cells were then transferred to a solution containing 0.1% CaCl 2 Sucrose medium (2% sucrose, 0.1% yeast powder, 0.1% peptone, 0.05% magnesium sulfate, 0.1% NaH 2 PO 4 , 0.1% KNO 3 , pH 6.6), cultured overnight at 30°C with shaking (150 rpm) to activate the immobilized cells. The immobilized cells were loaded into a 500 ml packed reactor, and 20% sucrose solution was added at different flow rates under the condition of 30° C., and the concentration of isomaltulose in the effluent was detected. When the sucrose flow rate was 4 ml / min, the sucrose conversion rate was 99.9%, and the concentrations of isomaltulose, mur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for directly producing isomaltulose by condensation crystallization without a separating purification step. The method is characterized in that: in the process of preparing the isomaltulose, the complex processes of separating and purifying the isomaltulose by ion exchange resin and the like are omitted in industrialized production, and the vacuum condensation and crystallization of conversion solution of sucrose are directly performed to obtain the purified isomaltulose. In the method, cell debris containing activity of sucrose isomerase is firstly prepared into a biocatalyst, the sucrose is subjected to biological conversion by catalysis to generate a product of the isomaltulose not containing byproducts such as glucose, fructose and the like, and the conversion solution of sucrose is directly subjected to vacuum condensation, crystallization and drying to produce the crystallized product of the isomaltulose. The method has the advantages of greatly lowering production cost, saving a large amount of process water and simplifying the production process.

Description

technical field [0001] The invention relates to a production technology for preparing isomaltulose by using sucrose as a raw material, and a production process for isomaltulose without separating and removing by-products such as glucose and fructose during the production process of isomaltulose. Background technique [0002] The chemical name of isomaltulose is α-D-glucopyranoside-1,6-D-fructofuranose, which was first found in honey. Because its content is very small, it has not attracted people's attention. The importance of isomaltulose was not gradually recognized until 1957, when this "new" disaccharide compound, isomaltulose, was discovered and crystallized during sugar beet production. Subsequently, the discovery that certain bacteria could convert sucrose into isomaltulose led to extensive research on the edibility and production possibilities of isomaltulose. For those special groups who are not suitable for sugar intake and need to carefully choose sweeteners, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12P19/24C12P19/12C12R1/22
Inventor 李宪臻
Owner DALIAN POLYTECHNIC UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap