Method for preparing wheat esterase biological sensor

Abstract

The invention discloses a method for preparing a wheat esterase biological sensor, which relates to a method for preparing a wheat esterase biological sensor. The invention solves the problem that the existing enzyme biological sensor is large and expensive, has complex operations and needs professional operators to operate, thus being not applicable to regular spot inspection. The method for preparing the wheat esterase biological sensor comprises the steps: firstly, the neutral salt extraction method is adopted to extract wheat esterase from wheat; ammonium sulfate salting out method and column chromatography are used for separating and purifying the crude enzyme extract; the sol-gel process is adopted to immobilize the separated and purified wheat esterase so as to prepare fixed wheat esterase; and the immobilized wheat esterase is connected with an energy exchanging element to form a biological sensor for detecting organophosphorus pesticides. The wheat esterase biological sensor prepared by the invention does not need to be connected with an external power source or use a reference electrode, but has the advantages of small volume, light weight, quick response speed, electromagnetic interference resistance, corrosion resistance, relatively high sensitivity, relatively high measuring bandwidth, convenient operation, low cost and the like. The method is applicable to quick detection of organophosphorus pesticides in food.

Description

technical field [0001] The invention relates to a preparation method of a wheat esterase biosensor. Background technique [0002] In the process of agricultural development in the world today, although green and organic have become themes, modern agriculture still largely relies on some negative effects, such as the use of pesticides. Organophosphorus pesticides have the advantages of many varieties, high efficacy, and wide range of control, and are widely used in agricultural production in my country. However, due to the high toxicity of organophosphorus pesticides and their residues, long-term intake of organophosphorus pesticides can cause a series of pathological changes such as decreased liver function, increased blood sugar, and decreased phagocytosis of leukocytes, as well as teratogenic, carcinogenic, and mutagenic effects. Severe cases can lead to central nervous system dysfunction, so organophosphorus pesticide residues have become a major food safety problem in C...

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that existing enzyme biosensors have huge equipment, expensive equipment, complicated procedures, and require professionals to operate, so they are not suitable for frequent on-site detection, etc., the present invention provides a preparation method for wheat esterase biosensors, which can Effectively improve the speed and accuracy of the determination of organophosphorus pesticide residues in food, and reduce the cost of biosensors