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Creatinine urine test paper and preparing method thereof

A urinalysis and test strip technology, applied in biological testing, material testing products, etc., can solve the problems of high measured value and large interference of creatinine by salt, and achieve the effects of convenient use, strong resistance to salt interference, and easy operation.

Active Publication Date: 2009-11-25
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a creatinine urine analysis test paper and a preparation method to solve the problem that the current copper method is used to test creatinine due to the large salt interference and the high measured value

Method used

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  • Creatinine urine test paper and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Liquid A:

[0021] 2M sodium hydroxide buffer, pH: 10.5 1000ml

[0022] Sodium chloride 2g

[0023] Polyvinylpyrrolidone 1g

[0024] Orange G 100mg

[0025] Liquid B:

[0026] 3.5-Dinitrobenzoic acid 400mg

[0027] Methanol 1000ml

[0028] Mix and dissolve liquid A and liquid B respectively, soak the above solution with 3MM filter paper, then dry the 3MM filter paper in an oven at 80°C for 30 minutes; then soak the 3MM filter paper again The above solution B was dried at a temperature of 50° C. for 25 minutes.

Embodiment 2

[0030] Liquid A:

[0031] 2M sodium hydroxide buffer, pH: 11.5 1000ml

[0032] Sodium chloride 5g

[0033] Polyvinylpyrrolidone 7g

[0034] Orange G 150mg

[0035] Liquid B:

[0036] 3.5-Dinitrobenzoic acid 400mg

[0037] Methanol 1000ml

[0038] Mix and dissolve liquid A and liquid B respectively, soak the above solution with 3MM filter paper, then dry the 3MM filter paper in an oven at 90°C for 25 minutes; then soak the 3MM filter paper again The above solution B was dried at a temperature of 55°C for 20 minutes.

Embodiment 3

[0040] Liquid A:

[0041] 2M sodium hydroxide buffer solution (pH: 12.5) 1000ml

[0042] Sodium chloride 3.5g

[0043] Polyvinylpyrrolidone 4g

[0044] Orange G 200mg

[0045] Liquid B:

[0046] 3.5-Dinitrobenzoic acid 600mg

[0047] Methanol 1000ml

[0048] Mix and dissolve liquid A and liquid B respectively, soak the above solution with 3MM filter paper, then dry the 3MM filter paper in an oven at 100°C for 20 minutes; then soak the 3MM filter paper again The above solution B was dried at a temperature of 60°C for 15 minutes.

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Abstract

The present invention relates to a creatinine urine test paper which belongs to an in vitro diagnostic reagent. The creatinine urine test paper comprises the following components: 1000ml of 2M sodium hydroxide buffer solution with the pH value of 10.5-12.5, 2g-5g of sodium chloride or potassium chloride, 1g-7g of polyvinyl pyrrolidone, 100mg-200mg of orange G; 400mg-800mg of 3,5-dinitrobenzonic acid, and 1000ml of methanol. The creatinine urine of the invention has the following advantages: convenient use, excellent reliability, simple preparing method, simple operation and strong salt interference resistance.

Description

technical field [0001] The invention is a urinalysis test strip, which belongs to in vitro diagnostic reagents. Background technique [0002] Creatinine in the body includes exogenous creatinine ingested from food and endogenous creatinine produced in the body. The daily production of endogenous creatinine in the same individual is relatively constant. Since creatinine is a small molecular weight terminal metabolite that does not bind to plasma proteins, except for a small amount that is excreted by ion channels in the renal tubules, most of it enters the primary urine through glomerular filtration and is not reabsorbed by the renal tubules. If the intake of exogenous creatinine can be controlled, creatinine can be used as an ideal endogenous substance for clearance rate examination. Therefore, the detection of changes in urine creatinine content has important reference value for the diagnosis of kidney disease, observation of disease changes and treatment effects. [000...

Claims

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Application Information

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IPC IPC(8): G01N33/52
Inventor 顾小丰
Owner DIRUI MEDICAL TECH CO LTD
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