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Method for embedding and protecting bifidobacteria by lactalbumin epoxy resin

A technology of whey protein and bifidobacteria, which is applied in food forming, food preparation, food science, etc., can solve the problems of staying in the experimental stage, loss of protection, and unsuitable application of liquid products, etc., to achieve good embedding, good protective effect

Inactive Publication Date: 2009-12-09
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research at this stage is simply pursuing the survival rate of the bacteria and ignoring the product cost and the feasibility of industrial production, resulting in most of the experiments only staying in the experimental stage, and it is difficult to put them into the market
Moreover, the development of probiotic microcapsules is mainly focused on solid powder products. These products lose their protective effect on the bacteria in liquid products due to the partial or complete dissolution of the wall material in liquid products, so the solid powder However, the development of microcapsule products suitable for liquid products started relatively late, so a lot of basic research and development and application research are still needed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031]The method for wrapping and protecting bifidobacterium in whey protein cold gel comprises: dispersing whey protein, denaturation treatment, mixing, and gel formation, and is characterized in that: the mixing is adding bifidobacteria in a sterile environment bacilli, and then mix them evenly. The gel formation is to add a gelling agent to the whey protein denaturation solution containing bacteria after mixing, place it on a stirrer, and stir in a constant temperature water bath at 25-35°C for 3.5 ~4 hours, and then placed at 4° C. for overnight treatment again, the formed product is cryogel of whey protein containing bifidobacteria (CGWPCB).

Embodiment 2

[0033] The method for protecting bifidobacteria by wrapping and protecting bifidobacteria in the whey protein cold gel, the dispersing whey protein is to disperse the whey protein isolate in sterilized distilled water according to the mass concentration of 5-10% w / w, Stir slowly at room temperature for 1 to 2 hours; the denaturation treatment is to place the whey protein solution in a water bath at a constant temperature of 70 to 80°C for 25 to 35 minutes, then quickly place it in an ice bath to cool, and wait until the temperature drops to room temperature Place in refrigerator overnight at 4°C.

Embodiment 3

[0035] In the method for protecting bifidobacteria by wrapping whey protein cold gel, in the gel formation process, salt induces the formation of whey protein cold gel CaCl 2 The final concentration of glucono-δ-lactone ranges from 6.0 to 10.0 mmol / L, and the final concentration of glucono-δ-lactone ranges from 0.15 to 0.50% w / w during acid-induced whey protein cryogel formation.

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Abstract

The invention relates to a method for embedding and protecting bifidobacteria by lactalbumin epoxy resin. The bifidobacteria are gram positive physiological bacteria which are transplanted in bodies of humans or mammals and are intestinal preponderant flora. The bifidobacteria have the probiotic functions of maintaining the balance of the intestinal flora, stimulating the immune function of a body, preventing diseases of a digestive tract, promoting digestion and absorption, relieving lactose intolerance and reducing serum cholesterase and have anticancer and antioxidative functions. The method comprises the steps: lactoalbumin dispersion, denaturation treatment, mixture and gel formation; in the mixture step, the bifidobacteria are added into a sterile environment and then are evenly mixed; in the gel formation step, after gelata is added into an evenly mixed lactoalbumin denaturation solution with thalli, the solution is placed on a stirrer, is stirred for 3.5 hours to 4 hours in a thermostatic water bath from 25 DEG C to 35 DEG C and is placed under a condition of 4 DEG C again to carry out overnight treatment, and a formed product is the lactalbumin epoxy resin with the bifidobacteria. The method is used for embedding and protecting the bifidobacteria by the lactalbumin epoxy resin.

Description

Technical field: [0001] The invention relates to a method for wrapping and protecting bifidobacteria with whey protein cold gel. Background technique: [0002] Bifidobacterium is a gram-positive physiological bacterium that colonizes humans or some mammals, and is the dominant intestinal flora. It belongs to the original flora in microecology and is of great significance to maintaining the health of the body. The probiotic effects of bifidobacteria mainly include maintaining the balance of intestinal flora, stimulating the immune function of the body, preventing digestive tract diseases, promoting digestion and absorption, alleviating lactose intolerance, lowering serum cholesterol, and anti-cancer and anti-oxidation effects. [0003] The practical application of bifidobacteria is severely limited due to its high nutritional requirements, extreme sensitivity to oxygen, poor resistance to low pH, and difficulty in maintaining activity. The most critical problem existing in i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23P1/04A23L33/00A23P10/30
Inventor 孟祥晨张久龙
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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