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Field identification method for peanut aspergillus flavus infection resistance

An identification method, the technology of Aspergillus flavus, which is applied in the agricultural field, can solve the problems of unstable resistance detection and difficult identification of resistance to Aspergillus flavus infection, and achieve the effect of low cost, high stability and accurate identification results

Active Publication Date: 2009-12-16
SHANDONG PEANUT RES INST
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AI Technical Summary

Problems solved by technology

[0005] (Purpose of the invention) The purpose of the present invention is to solve the problems such as the difficulty in identifying the resistance to Aspergillus flavus infection in the field of peanut varieties, the instability of resistance detection, etc.

Method used

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  • Field identification method for peanut aspergillus flavus infection resistance

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Embodiment Construction

[0018] The peanut varieties to be identified are planted in a cement pool with a shed cover, and the surrounding and bottom of the cement pool are sealed with cement, filled with medium loam, and planted with peanut cultivar J11 that is resistant to Aspergillus flavus infection and is resistant to Aspergillus flavus infection. Infected peanut cultivar Jinhua 1012 (Arachis hypogaea L.);

[0019] Corn crushed manually or by machine, 3-5mm in diameter, 50g-75g corn / m indoors 2 Peanuts are inoculated with Aspergillus Flavus to produce Aspergillus Flavus spores;

[0020] Watering was stopped 6 weeks before peanut harvest, and rain was artificially controlled. Before inoculation, make a ditch between the rows of peanuts, with a depth of about 2-3cm. 35 days, 25 days, and 15 days before the peanuts are harvested, sprinkle Aspergillus flavus spores in the ditch between the rows, and cover with soil 2-3mm;

[0021] Use a watering can according to 100g-150g / m 2 Spray water evenly on ...

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Abstract

The invention provides a field identification method for peanut aspergillus flavus infection resistance, which comprises the following steps: 1) crushing corn until the diameter thereof is between 3 and 5 millimeters, and producing aspergillus flavus spores according to 50 to 75 grams of corn / m<2> of peanut inoculation aspergillus flavus; 2) stopping watering 5 to 6 weeks before harvesting peanuts, making ditches of which the depth is about 2 to 3 centimeters among peanut rows before the inoculation, broadcasting the aspergillus flavus spores in the ditches among the rows for three times before harvesting the peanuts, and covering the ditches with soil for 2 to 3 millimeters; and 3) using a watering can to water the near-earth surface evenly after the inoculation according to 100 to 150 grams / m<2> to create an environment of which the temperature and the moisture are suitable for the propagation of the aspergillus flavus. Compared with the prior art, the method is the most similar field peanut growing environment, and has accurate identification result and high stability; and the used devices can be used for a plurality of years with only once investment, and generate no adverse effects on environmental pollution and human body health.

Description

field of invention [0001] The invention relates to the field of agricultural technology, in particular to a field identification method for peanut resistance to Aspergillus flavus infection. Background technique [0002] Peanut is the main oil crop and economic crop in China. my country's peanut production, total production and export volume rank first. The peanut planting area accounts for 19% of the world's total planting area, ranking second in the world, and its export trade accounts for 50% of the world. Ranking first among the major peanut exporting countries in the world, the average annual output accounts for 41% of the world's total output. my country is the country that produces and exports the most peanuts in the world. [0003] In recent years, aflatoxin pollution has severely restricted the benign and sustainable development of my country's peanut industry, and has become a worldwide problem that seriously affects peanut production, processing, trade and food saf...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/67
Inventor 单世华闫彩霞陈高孙兵李春娟张廷婷
Owner SHANDONG PEANUT RES INST
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