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Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof

A bifidobacterium and starter technology, applied in the field of bifidobacteria deep-frozen direct-injection starter and its compound cryoprotectant, can solve the problems of low survival rate and decreased vigor of strains, and achieve good stability and maintain good vigor Effect

Active Publication Date: 2009-12-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of low survival rate and decreased strain viability during the preparation of conventional bifidobacterium starter

Method used

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  • Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof
  • Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof
  • Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-24

[0029] (1) Strain cultivation: the preserved Bifidobacterium longum BBMN68 (preserved in China Agricultural University) was subcultured twice to rejuvenate, the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in corn steep liquor dry powder medium and cultured anaerobically at constant pH 7.0 for 24 hours.

[0030] Corn steep liquor dry powder medium is prepared from the following components: corn steep liquor dry powder 55g / L, glucose 10g / L, L-cysteine ​​hydrochloride 0.05g / L, A liquid 10mL / L, B liquid 5ml / L.

[0031] The A liquid is composed of the following components: KH 2 PO 4 100g / L, K 2 HPO 4 100g / L.

[0032] The B liquid is composed of the following components: MgSO 4 .7H 2 O 2g / L, FeSO 4 .7H 2 O 2g / L, NaCl 2g / L, MnSO 4 1.3g / L.

[0033] (2) Collect the bacteria: centrifuge the bacteria liquid obtained in (1) at 4000 rpm for 10 minutes to obtain the bacteria, and...

Embodiment 25

[0043] Preparation and Application of Example 25 Bifidobacterium Deep Freezing Fermentation Agent

[0044] (1) Strain cultivation: the preserved Bifidobacterium longum BBMN68 (preserved in China Agricultural University) was subcultured twice to rejuvenate, the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in corn steep liquor dry powder medium and cultured anaerobically at constant pH 7.0 for 24 hours.

[0045] Corn steep liquor dry powder medium is prepared from the following components: corn steep liquor dry powder 55g / L, glucose 10g / L, L-cysteine ​​hydrochloride 0.05g / L, A liquid 10mL / L, B liquid 5ml / L.

[0046] The A liquid is composed of the following components: KH 2 PO 4 100g / L, K 2 HPO 4 100g / L.

[0047] The B liquid is composed of the following components: MgSO 4 .7H 2 O 2g / L, FeSO 4 .7H 2 O 2g / L, NaCl 2g / L, MnSO 4 1.3g / L.

[0048] (2) Collect the bacteria...

Embodiment 26

[0059] Preparation and Application of Example 26 Bifidobacterium Deep Freezing Fermentation Agent

[0060] (1) Strain cultivation: the preserved Bifidobacterium adolescentis BBMN23 (preserved in China Agricultural University) was subcultured twice to rejuvenate, and the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in MRS medium and cultured anaerobically at constant pH 6.5 for 18.

[0061] MRS medium consists of the following components: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, sodium acetate 5g / L, diammonium citrate 2g / L, KH 2 PO 4 2g / L, Tween 80 1g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.28g / L, cysteine ​​hydrochloride 0.5g / L.

[0062] (2) Collect the bacteria: centrifuge the bacteria liquid obtained in (1) at 4500 rpm for 8 minutes to obtain the bacteria, and collect the bacteria sludge.

[0063] (3) Add protective agent:

[0064] B...

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Abstract

The invention discloses a bifidobacterium deep freezing starter culture and a cryoprotectant thereof. Bifidobacterium thallus is obtained by high-density culture, bacterial sludge is collected by centrifugation, the thallus is resuspended by utilizing a preferential complex protective agent, and the deep freezing preservation is carried out at minus 80 DEG C, thereby effectively keeping the bacterial counts and the functional properties of bifidobacterium. The bifidobacterium deep freezing starter culture is taken as an auxiliary starter culture which has significant effect on effectively applying probiotics in fermentation of dairy products.

Description

technical field [0001] The invention relates to a bifidobacterium deep-layer freezing direct-throwing starter and a composite cryoprotectant thereof. Background technique [0002] As a classic probiotic, the probiotic function of bifidobacteria has been widely recognized, and its application in functional foods has also received extensive attention, among which the application in dairy products is the most representative. However, bifidobacteria have various disadvantages in fermentation performance and production performance. First of all, Bifidobacteria are extremely sensitive to the external environment, and the survival rate of Bifidobacteria is low when they experience low temperature, dryness, and other adversity stimuli during the preparation of the starter; secondly, Bifidobacteria are obligate anaerobic bacteria and have high requirements for fermentation conditions. The rate of acid production by aerobic fermentation is low, and the flavor is poor; it is difficult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/123
Inventor 刘松玲任发政蒋菁莉刘爱萍赵亮张明杨海莺田寒友
Owner CHINA AGRI UNIV
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