Bifidobacterium deep freezing direct vat starter culture and composite cryoprotectant thereof
A bifidobacterium and starter technology, applied in the field of bifidobacteria deep-frozen direct-injection starter and its compound cryoprotectant, can solve the problems of low survival rate and decreased vigor of strains, and achieve good stability and maintain good vigor Effect
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Embodiment 1-24
[0029] (1) Strain cultivation: the preserved Bifidobacterium longum BBMN68 (preserved in China Agricultural University) was subcultured twice to rejuvenate, the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in corn steep liquor dry powder medium and cultured anaerobically at constant pH 7.0 for 24 hours.
[0030] Corn steep liquor dry powder medium is prepared from the following components: corn steep liquor dry powder 55g / L, glucose 10g / L, L-cysteine hydrochloride 0.05g / L, A liquid 10mL / L, B liquid 5ml / L.
[0031] The A liquid is composed of the following components: KH 2 PO 4 100g / L, K 2 HPO 4 100g / L.
[0032] The B liquid is composed of the following components: MgSO 4 .7H 2 O 2g / L, FeSO 4 .7H 2 O 2g / L, NaCl 2g / L, MnSO 4 1.3g / L.
[0033] (2) Collect the bacteria: centrifuge the bacteria liquid obtained in (1) at 4000 rpm for 10 minutes to obtain the bacteria, and...
Embodiment 25
[0043] Preparation and Application of Example 25 Bifidobacterium Deep Freezing Fermentation Agent
[0044] (1) Strain cultivation: the preserved Bifidobacterium longum BBMN68 (preserved in China Agricultural University) was subcultured twice to rejuvenate, the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in corn steep liquor dry powder medium and cultured anaerobically at constant pH 7.0 for 24 hours.
[0045] Corn steep liquor dry powder medium is prepared from the following components: corn steep liquor dry powder 55g / L, glucose 10g / L, L-cysteine hydrochloride 0.05g / L, A liquid 10mL / L, B liquid 5ml / L.
[0046] The A liquid is composed of the following components: KH 2 PO 4 100g / L, K 2 HPO 4 100g / L.
[0047] The B liquid is composed of the following components: MgSO 4 .7H 2 O 2g / L, FeSO 4 .7H 2 O 2g / L, NaCl 2g / L, MnSO 4 1.3g / L.
[0048] (2) Collect the bacteria...
Embodiment 26
[0059] Preparation and Application of Example 26 Bifidobacterium Deep Freezing Fermentation Agent
[0060] (1) Strain cultivation: the preserved Bifidobacterium adolescentis BBMN23 (preserved in China Agricultural University) was subcultured twice to rejuvenate, and the strain morphology of the culture solution was examined under a microscope, and the activated strain was divided into 10 7 Cfu / mL was inoculated in MRS medium and cultured anaerobically at constant pH 6.5 for 18.
[0061] MRS medium consists of the following components: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, sodium acetate 5g / L, diammonium citrate 2g / L, KH 2 PO 4 2g / L, Tween 80 1g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.28g / L, cysteine hydrochloride 0.5g / L.
[0062] (2) Collect the bacteria: centrifuge the bacteria liquid obtained in (1) at 4500 rpm for 8 minutes to obtain the bacteria, and collect the bacteria sludge.
[0063] (3) Add protective agent:
[0064] B...
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