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Method for extracting alkaloid from seabuckthorn seeds and application of active component of alkaloid to angiocardiopathy prevention

An active ingredient and technology of sea buckthorn seeds is applied in the field of extraction and separation of active ingredients of sea buckthorn seeds alkaloids, which can solve the problems of little research and achieve the effects of good effect, good anti-myocardial ischemia activity and protective effect.

Inactive Publication Date: 2009-12-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no domestic report on the research on the anti-myocardial ischemic injury of seabuckthorn seed ethanol extract, and there are few related researches abroad

Method used

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  • Method for extracting alkaloid from seabuckthorn seeds and application of active component of alkaloid to angiocardiopathy prevention
  • Method for extracting alkaloid from seabuckthorn seeds and application of active component of alkaloid to angiocardiopathy prevention
  • Method for extracting alkaloid from seabuckthorn seeds and application of active component of alkaloid to angiocardiopathy prevention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050]Put 1.0kg of seabuckthorn seed meal in a 10-liter conical flask, extract with 6 liters of 10% ethanol at 70°C for 1 hour, centrifuge to separate the extract, add 6 liters of 10% ethanol to the residue, and stir and extract for 2 hours. The extraction temperature was 70°C, and the extraction was performed three times. The residue was removed by centrifugation and concentrated. Add 3 times the volume of ethyl acetate to the concentrated solution with stirring, then stand still for 5 hours, fractionate the ethyl acetate, then add 3 times the volume of ethyl acetate with stirring, then stand still for 6 hours, fractionate the ethyl acetate. Repeat several times until the ethyl acetate phase is colorless. Combine the ethyl acetate phases and concentrate under reduced pressure below 65°C until there is no ethyl acetate smell, so as to obtain the crude extract of seabuckthorn seed alkaloids.

[0051] Put the seabuckthorn seed alkaloid crude extract on a macroporous resin (mod...

Embodiment 2

[0053] Put 10kg of seabuckthorn seed meal in a 100-liter extraction tank, extract with 60 liters of 70% ethanol at 30°C for 1 hour, centrifuge to separate the extract, add 60 liters of 70% ethanol to the residue, and extract at 30°C Stir and extract for 2 hours, centrifuge to remove the residue, and extract three times in total. The combined supernatants were concentrated. Add 2 times the volume of ethyl acetate to the concentrated solution with stirring, then stand still for 5 hours, fractionate the ethyl acetate, then add 3 times the volume of ethyl acetate with stirring, then stand still for 6 hours, fractionate the ethyl acetate. Repeat several times until the ethyl acetate phase is colorless. Combine the ethyl acetate phases and concentrate under reduced pressure below 65°C until there is no ethyl acetate smell, so as to obtain the crude extract of seabuckthorn seed alkaloids.

[0054] Put the seabuckthorn seed alkaloid crude extract on a macroporous resin (model AB-8) ...

Embodiment 3

[0056] Put 50kg of seabuckthorn seed meal in a 500-liter extraction tank, extract with 300 liters of 50% ethanol at 50°C for 1 hour, centrifuge to separate the extract, add 300 liters of 50% ethanol to the residue, and extract at 50°C Stir and extract for 2 hours, centrifuge to remove the residue, and extract three times in total. The combined supernatants were concentrated to one-tenth volume. Add 2 times the volume of ethyl acetate to the concentrated solution under stirring, then stand still for 5 hours, distill off ethyl acetate, then add 3 times the volume of ethyl acetate under stirring, then stand still for 6 hours, fractionally distill off ethyl acetate. Repeat several times until the ethyl acetate phase is colorless. Combine the ethyl acetate phases and concentrate under reduced pressure below 65°C until there is no ethyl acetate smell, so as to obtain the crude extract of seabuckthorn seed alkaloids.

[0057] Put the seabuckthorn seed alkaloid crude extract on a ma...

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Abstract

The invention relates to a method for extracting alkaloid from seabuckthorn seeds and application of active component of alkaloid to angiocardiopathy prevention, and belongs to the technical field of development and utilization of seabuckthorn resources. The method comprises the following steps: A, taking seabuckthorn seeds as raw materials, extracting the seabuckthorn seeds with ethanol solution, merging and condensing extraction solution to obtain seabuckthorn seed extractive concentrated solution; B, taking the seabuckthorn seed extractive concentrated solution, extracting the solution with ethylacetate, distilling the ethylacetate until the ethylacetate is colorless; merging, decompressing and condensing the ethylacetate to obtain seabuckthorn seed alkaloid crude extracts; and C, loading the seabuckthorn seed alkaloid crude extracts onto AB-8 macroporous resin column, washing the eabuckthorn seed alkaloid crude extracts with water which is one time of the AB-8 macroporous resin column firstly; eluting the eabuckthorn seed alkaloid crude extracts with the ethanol solution; and collecting and condensing the ethanol elution; and obtaining seabuckthorn seed alkaloid composition. Active components of the extracted seabuckthorn seed alkaloid are used for preventing angiocardiopathy; the cardiac muscle cell anoxia and reoxgenation cause ischemic damage; after the seabuckthorn seed alkaloid is used to intervene, the survival rate of cardiac muscle cell is remarkably increased so as to reduce the occurrence of the angiocardiopathy.

Description

technical field [0001] The invention relates to a method for extracting and isolating active components of seabuckthorn seed alkaloids and the application of the anti-cardiovascular disease activity. The invention belongs to the technical field of development and utilization of seabuckthorn plant resources. Background technique [0002] Seabuckthorn seeds are the seeds of the fruit of the plant of the genus Hippophae in the family Elaeanaeeae. Seabuckthorn has the function of anti-oxidation and improving immunity. It is reported that the ethanol extract of seabuckthorn seeds can increase the activity of superoxide dismutase (SOD) in mouse erythrocytes and the activity of glutathione peroxidase (GSH-Px) in mouse whole blood, and can reduce the lipid peroxide in mouse liver. quality content; the ethanol extract of seabuckthorn seeds can remove HO, inhibit the production of malondialdehyde caused by HO, reduce the hemolysis of red blood cells caused by HO, reduce the degree o...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P9/00A61P9/10A61K131/00
Inventor 徐德平胡长鹰唐年初赵晨伟梅金龙
Owner JIANGNAN UNIV
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