High-polymer three-dimensional amino-group substrate as well as preparation method and application thereof

Abstract

The invention discloses a high-polymer three-dimensional amino-group substrate as well as a preparation method and the application thereof. The method for preparing the biochip substrate comprises the following step: connecting amino-enriching polymer on the surface of a slide substrate to obtain a substrate with the amino-enriching polymer being connected on the surface. Experiments prove that the substrate has greatly-improved bimolecular fixing sensitivity, satiated sample points and more stable surface property, and ensures that the shapes of the sample points in the process of long-time sample pointing always keep uniform, and the non-specific adsorption and the self-fluorescence background of the sample points are both very low. The substrate is suitable for the pointing high-density chips which are pointed for a long time, and solves the problem that a major of samples of the high-density chips pointed for a long time can not be fixed in the common amino substrate. The substrate is a biochip substrate material with excellent performance and can be widely applied on the preparation of various biochips.

Description

technical field [0001] The invention relates to a high molecular polymer three-dimensional amino-based sheet and its preparation method and application. Background technique [0002] As an important means of obtaining relevant information efficiently and on a large scale, biochip technology is a synthesis of biotechnology, medical technology, micro-processing technology, microelectronics technology, and material technology, and the application of material technology is concentrated in the biochip substrate ( carrier), that is, the preparation of the substrate. The substrate is the basis of the chip, and the preparation of the substrate is particularly important. [0003] At present, the silanized amino substrate commonly used in spotting chips has stability problems in spotting high-density chips. In the spotting environment for a long time, the spot diameter is normal at the beginning of spotting, and most of the dot diameters are getting smaller after that. The phenomeno...

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Problems solved by technology

[0003] At present, the silanized amino substrate commonly used in spotting chips has stability problems in spotting high-density chips. In the spotting environment for a long time, the spot diameter is normal at the beginning of spotting, and most of the dot diameters are getting smaller after that. The phenomenon of getting smaller and smaller, that is, the phenomenon of stars and stars, causes the signal in the area where the sample is first sampled after chip hybridization to be normal, and most of the points thereafter basically have no signal, and the longer the sample is, the more serious this problem is.

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