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Sudan hapten and antigen as well as preparation method and application thereof

A reaction and antibody technology, which is applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problems of cumbersome operation steps, high cost, and inapplicability to large-scale sample screening and detection, and achieve broad application prospects , high accuracy, and simple sample pretreatment

Inactive Publication Date: 2010-02-10
北京维德维康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have strong specificity and high sensitivity, the sample pretreatment steps are cumbersome and costly, and they are not suitable for screening and detection of large batches of samples.

Method used

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  • Sudan hapten and antigen as well as preparation method and application thereof
  • Sudan hapten and antigen as well as preparation method and application thereof
  • Sudan hapten and antigen as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the preparation of Sudan Red Hapten and Sudan Red Antigen

[0042] The preparation process of Sudan red hapten and Sudan red antigen is as follows: figure 1 shown.

[0043] 1. Synthesis of Sudan Red Hapten

[0044] (1) Synthesis

[0045] 1. Diazotization reaction: Dissolve m-aminobenzoic acid in concentrated hydrochloric acid, add an aqueous solution containing sodium nitrite at 5°C, stir and react for 28 minutes to obtain a light yellow diazonium salt solution, which is represented by formula III A solution of the diazide ion of m-aminobenzoic acid.

[0046] The molar ratio of m-aminobenzoic acid, concentrated hydrochloric acid and sodium nitrite is 1:2:1.

[0047]

[0048]2. Coupling reaction: 2-naphthol is dissolved in 20% (mass percentage composition) sodium hydroxide (the amount ratio of 2-naphthol and the feeding material of formula III diazonium ion is 1: 1), is cooled to At 5°C, slowly add m-aminobenzoic acid diazide ion solution, and reac...

Embodiment 2

[0057] Embodiment 2, the preparation of Sudan Red Hapten and Sudan Red Antigen

[0058] The preparation process of Sudan red hapten and Sudan red antigen is as follows: figure 1 shown.

[0059] 1. Synthesis of Sudan Red Hapten

[0060] (1) Synthesis

[0061] 1. Diazotization reaction: Dissolve m-aminobenzoic acid in concentrated hydrochloric acid, add an aqueous solution containing sodium nitrite at 8°C, and stir for 25 minutes to obtain a light yellow diazonium salt solution, which is represented by formula III A solution of the diazide ion of m-aminobenzoic acid.

[0062] The molar ratio of m-aminobenzoic acid, concentrated hydrochloric acid and sodium nitrite is 1:1.5:2.5.

[0063]

[0064] 2. Coupling reaction: 2-naphthol is dissolved in 20% (mass percentage composition) sodium hydroxide (the ratio of the feed material of 2-naphthol and formula III diazonium ion is 1: 1.2), is cooled to Slowly add m-aminobenzoic acid diazide ion solution at 5°C, and react at 8°C f...

Embodiment 3

[0072] Embodiment 3, the preparation of Sudan red hapten and Sudan red antigen

[0073] The preparation process of Sudan red hapten and Sudan red antigen is as follows: figure 1 shown.

[0074] 1. Synthesis of Sudan Red Hapten

[0075] (1) Synthesis

[0076] 1. Diazotization reaction: Dissolve m-aminobenzoic acid in concentrated hydrochloric acid, add an aqueous solution containing sodium nitrite at 10°C, and stir for 30 minutes to obtain a light yellow diazonium salt solution, which is represented by formula III A solution of the diazide ion of m-aminobenzoic acid.

[0077] The molar ratio of m-aminobenzoic acid, concentrated hydrochloric acid and sodium nitrite is 1.5:1:3.

[0078]

[0079] 2. Coupling reaction: 2-naphthol is dissolved in 20% (mass percentage composition) sodium hydroxide (the molar ratio of 2-naphthol and the feeding material of formula III diazonium ion is 1: 1.5), is cooled to 5°C, slowly add m-aminobenzoic acid diazide ion solution, and react at...

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Abstract

The invention discloses melamine hapten and antigen as well as a preparation method and application thereof. The preparation method comprises the following steps: 1) diazotizing m-aminobenzoic acid toobtain diazotized ions of the m-aminobenzoic acid shown in the formula III; 2) carrying out coupling reaction on the obtained diazotized ions of the m-aminobenzoic acid and 2-naphthol to obtain the compound shown in the formula I. The experimental results show that the titer of antiserum obtained by utilizing the antigen of the invention to immunize animals can reach 1:3*10<6>, the lowest limit of detection is 0.05ng / mL, the hemi-inhibitory concentration is 0.4ng / mL and the generated antibody has high specificity, high sensitivity and high accuracy. Compared with the existing chromatography,the method of using the antigen and / or antibody of the invention to detect Sudan has the characteristics of low cost, simple operation, easy sample preprocessing, convenience and rapidness and is suitable for large-scale detection. Therefore, the antigen, antibody and detection method of the invention have extensive application prospects.

Description

technical field [0001] The invention relates to a Sudan red hapten and antigen, a preparation method and application thereof. Background technique [0002] Sudan Red I, that is, 1-phenylazo-2-naphthol, the molecular formula is C 16 h 12 ON 2 , its solubility is less than 0.01g in the standard state, and it is easily soluble in organic solvents. It is a synthetic oil-soluble chemical dye. In 1896, the scientist Duddy named it Sudan Red and it is still used today. Sudan red is mainly used in petroleum, motor oil and some other industrial solvents to enhance its color, and it is also used to enhance the luster of shoes and floors. Because its chemical composition contains naphthalene ring and azo structure, it is a potential carcinogen, which can be reduced and degraded into aromatic amine compounds in different ways in the environment. Aniline has a strong carcinogenic effect on the human body, so Sudan Red Dyes cannot be used as food additives. When the content of Sudan ...

Claims

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Application Information

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IPC IPC(8): C07C245/10C07K14/765C07K14/77C07K1/113C07K16/44G01N33/566G01N33/531
Inventor 江海洋王战辉吴小平徐飞米铁军张静王进
Owner 北京维德维康生物技术有限公司
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