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Application of microRNA-146a in treatment of rheumatoid arthritis

A rheumatoid and arthritis technology, applied in the field of application of microRNA-146a in the treatment of rheumatoid arthritis, can solve problems such as unclear expression of miR-146a

Inactive Publication Date: 2010-02-10
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] MiR-146a is involved in the development and differentiation of immune cells, and there is also evidence that the expression of miR-146a is abnormal in peripheral blood mononuclear cells (PBMC) and synovial tissue of RA patients, but the significance of the abnormal expression of miR-146a is still unclear

Method used

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  • Application of microRNA-146a in treatment of rheumatoid arthritis
  • Application of microRNA-146a in treatment of rheumatoid arthritis
  • Application of microRNA-146a in treatment of rheumatoid arthritis

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1: Detection of miR-146a expression in PBMCs of RA patients

[0016] In this study, 30 RA patients who met the classification criteria of the American College of Rheumatology (ACR) in 1987 were included. At the same time, 30 age- and gender-matched normal volunteers were selected. Informed consent was obtained, and 4ml of anticoagulant blood was drawn. PBMC were separated immediately by density gradient centrifugation, total RNA was extracted by Trizol method, and A260 was measured by ultraviolet spectrophotometer. The relative expression level of MiR-146a was measured by ΔCt and 2 -ΔΔCt Calculated, U6snRNA was used as an internal reference, and the detection method was the same. Real-time quantitative PCR was performed according to the miRNAs Real-Time PCR Quantitation Kit provided by Shanghai Gemma Company. The results showed that the expression of miR-146a was significantly down-regulated in PBMCs of RA patients, as shown in Figure 1.

Embodiment 2

[0017] Example 2: Correlation analysis between the expression of miR-146a in PBMCs of RA patients and clinical indicators

[0018] In order to explore the clinical significance of the expression level of miR-146a, we conducted a Spearman correlation analysis between the expression level of miR-146a and various clinical indicators, and found that the expression level of miR-146a was negatively correlated with ESR, and no correlation with rheumatoid factor was found so far. (RF), anti-CCP antibody and other clinical indicators were correlated. See Figure 2.

Embodiment 3

[0019] Example 3: Correlation analysis of miR-146a expression in PBMCs of RA patients and target mRNA TRAF6, IRAK1

[0020] In order to understand the way miR-146a plays a role in RA, we also detected the expression levels of TRAF6 and IRAK1 in PBMCs of RA patients. TRAF6 and IRAK1 can activate NF-κb pathway to produce inflammatory factors such as TNF-α, and have been proved to be the targets of miR-146a. However, it is not clear whether miR-146a plays a regulatory role through TRAF6 and IRAK1 in RA. We detected the expression of TRAF6 and IRAK1 by qRT-PCR, and performed Spearman correlation analysis with miR-146a, and found that the expression of TRAF6 and IRAK1 was significantly up-regulated (Figure 3), and the contents of both were negatively correlated with miR-146a. Correlation (see Figure 4), indicating that miR-146a plays a regulatory role in RA at least partly by inhibiting the expression of TRAF6 and IRAK1.

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Abstract

The invention identifies low-expression microRNA (miR-146a) in RA (rheumatoid arthritis) inflammatory cells and confirms that the miR-146a has the effects of inhibiting RA synovial fibroblasts (RASFs)and inflammation.

Description

technical field [0001] The research involves the expression level of microRNA-146a (miR-146a) in peripheral blood mononuclear cells of rheumatoid arthritis, its pathway of action, and its inhibitory effect on synoviocyte function. Background technique [0002] Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic destructive joint disease. RA without proper treatment can persist and even lead to joint deformity. At present, the pathogenesis of RA is not clear, which limits the development of therapeutic methods. [0003] MicroRNA (microRNA, miRNA) is a kind of endogenous non-coding small molecule single-stranded RNA with a length of about 21 nt discovered in eukaryotes in recent years. It participates in post-transcriptional gene expression by binding to the 3'UTR of target mRNA. regulation. Current research shows that nearly 30% of human protein-coding genes are regulated by miRNAs. miRNAs not only participate in the normal development of or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11A61K48/00A61P19/04A61L29/00C12N15/113
Inventor 栗占国李茹龙丽
Owner PEOPLES HOSPITAL PEKING UNIV
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