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Method for preparing magnetic nano contrast agent and application thereof in pancreatic cancer magnetic resonance imaging

A technology of magnetic nanoparticles and magnetic nanoparticles, which is applied in the preparation of magnetic nano-contrast agents and the application of contrast agents in magnetic resonance imaging of pancreatic cancer, which can solve the problems of magnetic resonance imaging difficulties, lack of cell specificity, and hidden symptoms

Inactive Publication Date: 2010-02-17
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its unique anatomical location, hidden symptoms, and lack of cell-specific contrast agents, early magnetic resonance imaging of pancreatic cancer is difficult

Method used

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  • Method for preparing magnetic nano contrast agent and application thereof in pancreatic cancer magnetic resonance imaging
  • Method for preparing magnetic nano contrast agent and application thereof in pancreatic cancer magnetic resonance imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Ferric chloride (FeCl 3 ·6H 2 O), ferrous chloride (FeCl 2 4H 2 O) and the mass ratio of dextran three are 5: 1: 1, be dissolved in 100ml distilled water, form transparent and stable microemulsion system. Get 1ml concentration and be that 25% concentrated ammonia water (weight) is diluted with twice distilled water, it is slowly added in the microemulsion that stirs constantly, continues to stir for 10 minutes to make ammonia water evenly disperse in microemulsion. The temperature of the system was kept at 15° C. while stirring continuously for 2 hours; after the reaction was completed, the precipitate was removed by centrifugation, and excess dextran was separated and removed to finally obtain a stable aqueous solution of magnetic nanoparticles wrapped in dextran.

Embodiment 2

[0028] Ferric chloride (FeCl 3 ·6H 2 O), ferrous chloride (FeCl 2 4H 2 O) and the mass ratio of dextran three are 100: 10: 50, be dissolved in 100ml distilled water, form transparent and stable microemulsion system. Get 1ml concentration and be that 28% concentrated ammonia water (weight) is diluted with double-distilled water, it is slowly added in the microemulsion that stirs constantly, continues to stir for 30 minutes so that ammonia water is evenly dispersed in microemulsion. The temperature of the system was kept at 40° C. while stirring continuously for 5 hours; after the reaction was completed, the precipitate was removed by centrifugation, and excess dextran was separated and removed to finally obtain a stable aqueous solution of magnetic nanoparticles wrapped in dextran.

Embodiment 3

[0030] Ferric chloride (FeCl 3 ·6H 2 O), ferrous chloride (FeCl 2 4H 2 O) and the three mass ratios of dextran are 55:5:25 and are dissolved in 100ml distilled water to form a transparent and stable microemulsion system. Get 1ml concentration and be that 27% concentrated ammonia water (weight) is diluted with twice distilled water, it is slowly added in the microemulsion that stirs constantly, continues to stir for 20 minutes so that ammonia water is evenly dispersed in microemulsion. The temperature of the system was kept at 30° C. while stirring continuously for 4 hours; after the reaction was completed, the precipitate was removed by centrifugation, and excess dextran was separated and removed to finally obtain a stable aqueous solution of magnetic nanoparticles wrapped in dextran.

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Abstract

The invention discloses a method for preparing a contrast agent, in particular to a method for preparing a magnetic nano contrast agent and an application of the contrast agent in pancreatic cancer magnetic resonance imaging. The method comprises the following steps: taking the water solution of the magnetic nano particles wrapped by a right amount of glucan, adding a potassium periodate solutionand reacting for 5-10 hours in a way of keeping in a dark place; separating the solution and dispersing the separated particles in a boric acid buffer solution with the pH of 8.0; adding 10-50mul of rat resistance human CA19-9 antibody in the solution and reacting at the condition of 4 DEG C over night; then, adding a right amount of boron-potassium cyanide solution and reacting for 5-10 hours; and purifying and separating the mixture, then dispersing into a phosphoric acid buffer solution with the pH of 7.4 and obtaining the magnetic nano contrast agent. By the magnetic nano contrast agent provided by the invention, the obvious drop of an MRI signal can be represented in the application of the in-vitro magnetic resonance imaging of pancreatic cancer cells, thereby a useful basis is provided for the diagnosis of tumours.

Description

technical field [0001] The invention relates to a method for preparing a contrast agent, in particular to a method for preparing a magnetic nano contrast agent, and the application of the contrast agent in magnetic resonance imaging of pancreatic cancer. Background technique [0002] In recent years, magnetic resonance imaging technology has developed rapidly and has become increasingly mature and perfect. It has been widely used in the field of biomedicine and has become an indispensable and important tool in clinical diagnosis and basic research. Magnetic resonance imaging (MRI) is a new, non-invasive imaging method, which belongs to biological spin imaging technology and has no radiation influence. The spatial resolution of MRI images is high, and the relationship between anatomical structures is very clear. The information provided is not only more than that of many other imaging techniques in medical imaging, but also different from the existing imaging techniques. It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12
Inventor 沈鹤柏高明远黄明鸣缪飞
Owner SHANGHAI NORMAL UNIVERSITY