Use of isoliquiritigenin as medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors

A technology of isoliquiritigenin and malignant tumors, applied in the field of medicine, can solve the problems not involved in the application of isoliquiritigenin to inhibit tumor recurrence and metastasis, and achieve the effect of inhibiting tumor recurrence and metastasis with low cost

Inactive Publication Date: 2010-03-03
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent [an anti-tumor isoliquiritigenin tablet, application number: 200710053147.7] only discloses the process of preparing anti-tumor drug tablets with isoliquiritigenin, and does not involve the use of isoliquiritigenin in the preparation of prophylaxis or treatment of surgical resection Application of drugs to inhibit tumor recurrence and metastasis after tumor onset

Method used

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  • Use of isoliquiritigenin as medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors
  • Use of isoliquiritigenin as medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors
  • Use of isoliquiritigenin as medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0021] Experimental example 1 Effect of isoliquiritigenin on recurrence and metastasis of melanoma after surgical resection

[0022] Isoliquiritigenin was purchased from Jiangxi Bencao Tiangong Technology Co., Ltd., and its purity was 99.8% as determined by HPLC. The mouse B16F10 melanoma highly metastatic cell line was purchased from the China Center for Type Culture Collection. The cells were stored in DMEM medium containing 10% fetal bovine serum, 100 U / ml penicillin, and 100 μg / ml streptomycin at 37°C, 5% CO 2 Under the conditions of cultivation, the culture medium was changed every 24 hours, and the culture medium was subcultured every 3 days.

[0023] Take the cells in the logarithmic growth phase, digest with trypsin, and adjust the concentration to 1×10 with the above-mentioned complete culture solution. 7 / ml of single cell suspension, subcutaneously inject 0.1ml of tumor cell suspension into the left axilla of C57BL / 6 mice, and inoculate 150 mice in total. After 2 ...

experiment example 2

[0028] Experimental Example 2 Effect of Isoliquiritigenin on Recurrence and Metastasis after Surgical Resection of Primary Tumor of Lung Cancer

[0029] The mouse Lewis lung cancer cell line was purchased from Beijing Institute of Materia Medica, Chinese Academy of Medical Sciences, and the cells were inoculated subcutaneously in the axilla of C57BL / 6 mice. At the time of passaging, the tumor-bearing mice that had been inoculated with tumors for two weeks and had good tumor growth without ulceration were sacrificed by neck dislocation. After soaking in 75% alcohol for disinfection, the tumor pieces were aseptically stripped and cut into 1mm pieces. 3 Large and small lumps, in order of 20 #The puncture needle was inoculated subcutaneously in the axilla of C57BL / 6 mice, and a total of 150 mice were inoculated. After 2 weeks, 120 mice with tumor masses greater than 0.5 cm in length and diameter were selected and randomly divided into 6 groups, 20 mice in each group. One group w...

experiment example 3

[0034] Experimental example 3 Effect of isoliquiritigenin on recurrence and metastasis of human liver cancer after surgical resection of primary tumor

[0035] The experimental method refers to the literature [Xu Bing, Yao Ming, Gu Weiwang, et al. Establishment of an animal model of postoperative metastasis of human cancer and its biological characteristics. Journal of Southern Medical University. 2007; 27(7): 1009-11.].

[0036] The highly metastatic human liver cancer cell line LCI-D20 was purchased from the American Type Culture Collection (ATCC), and inoculated in SPF grade BALB / c-nu / nu nude mice for subcutaneous passage.

[0037] Take out subcutaneous xenografted tumors of tumor-bearing nude mice subcutaneously, immerse them in physiological saline containing 100 U / ml of penicillin and streptomycin, select tumor tissues that grow well, have no necrosis, and are fish-like, and cut them into about 1mm 3 The small pieces were transplanted subcutaneously into BALB / c-nu / nu nud...

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Abstract

The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors, which belongs to technical field ofmedicaments. The invention discloses the use of isoliquiritigenin as a medicament for preventing and treating or curing postoperative metastasis and relapse of malignant tumors. Researches show thatthe surgical resection of primary tumors can promote tumor metastasis. At present, no ideal therapeutic method or medicament for treating malignant tumor metastasis and relapse after the resection ofthe primary tumors is available. Experiments implemented by the inventor prove that the isoliquiritigenin has a remarkable inhibition effect on the relapse and metastasis of various malignant tumors after the surgical resection of the primary tumors. Based on the effect, the isoliquiritigenin can be used in clinic prevention or treatment of the relapse and metastasis of lung cancer, liver cancer,breast cancer, colorectal cancer and other various malignant tumors after the surgical resection of the primary tumors and has the obvious advantages of high safety, low price, good patient complianceand the like. The effect of the isoliquiritigenin may be related to the inhibition of the isoliquiritigenin on the self renewal and multiplication capacity of cancer stem cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new application of isoflavone compound extracted from licorice plants—isoliquiritigenin, in particular to its use as a drug for preventing or treating tumor recurrence and metastasis after surgical resection of malignant tumors Applications. Background technique [0002] Cancer, also known as malignant tumors or neoplasms, is a general term for more than 100 diseases that can occur in various parts of the body. The WHO defines cancer as abnormal cells that rapidly grow beyond their normal boundaries, capable of invading adjacent normal sites and spreading to other organs—a process called metastasis, which is the leading cause of cancer death[ http: / / www.who.int / mediacentre / factsheets / fs297 / en / , 2007]. [0003] Cancer is one of the leading causes of death in the world. In 2005, the number of deaths worldwide was about 58 million, of which 7.6 million died of canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/122A61P35/00
Inventor 王振华孙超郭雷刘军花王婷甘露张娟王祥飞李忌郑秋生
Owner SHIHEZI UNIVERSITY
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