An injectable bioactive hydrogel for tumor suppression and repair promotion

A technology of biologically active water and gel, which is applied in the field of tissue engineering, medicine, and biomedical materials, can solve problems such as application limitations, and achieve the effects of convenient operation, improved burst release, and inhibition of tumor recurrence

Active Publication Date: 2021-06-22
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether antineoplastic drugs and restorative drugs can exert their functions in the order of time and space, the clinical application is limited
[0008] In the field of biomaterials and medicine, problems involving multi-stage treatment after tumor resection have not been effectively resolved, and there is still a great demand for methods that simultaneously inhibit tumors and promote repair

Method used

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  • An injectable bioactive hydrogel for tumor suppression and repair promotion
  • An injectable bioactive hydrogel for tumor suppression and repair promotion
  • An injectable bioactive hydrogel for tumor suppression and repair promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of Hyaluronic Acid-Multifunctional Active Peptide JM2 Injectable Bioactive Hydrogel

[0045] Step 1. Dissolve 1 g of hyaluronic acid powder in 100 mL of deionized water and keep stirring to prepare a 1% hyaluronic acid solution. Place the hyaluronic acid solution at 4°C and pH=8.0, slowly add 7.4 mL of methacrylic anhydride solution dropwise into the hyaluronic acid solution and stir evenly. The above solution was stirred overnight at a low speed at 4°C and pH=8.0. The above solution was slowly poured into 1L of absolute ethanol, and precipitated overnight. Collect the precipitate, dialyze it through a dialysis bag (molecular weight cut-off 7kDa) for 7 days, collect the hyaluronic acid solution after dialysis and freeze-dry it for later use;

[0046] Step 2. Take 100 mg of the freeze-dried hyaluronic acid solution in step 1 and add 50 mL of triethanolamine buffer solution, and stir evenly. Add 37mg of multifunctional active peptide JM2 into the...

Embodiment 2

[0048] Example 2: Appearance and Microstructure Analysis of Hyaluronic Acid-Multifunctional Active Peptide JM2 Injectable Bioactive Hydrogel

[0049] Such as figure 1 As shown, the hyaluronic acid-multifunctional active peptide JM2 injectable bioactive hydrogel prepared in Example 1 has injectable properties and has a smooth appearance. The hyaluronic acid-multifunctional active peptide JM2 injectable bioactive hydrogel prepared in Example 1 was freeze-dried, and the dried slow-release system sample was cut to obtain a relatively flat internal cross-section, which was sprayed with gold on the surface and then scanned by emission Electron microscopy (SEM) was used to observe the cross-sectional structure of the active substance sustained-release system at an accelerating voltage of 10kV.

[0050] Such as figure 2 As shown, the hyaluronic acid-multifunctional active peptide JM2 injectable bioactive hydrogel has a hydrogel sheet structure inside under the emission scanning ele...

Embodiment 3

[0051] Example 3: Molecular properties of hyaluronic acid-multifunctional active peptide JM2 injectable bioactive hydrogel for sustained release active peptide JM2

[0052] Step a, take 50 mg of the lyophilized hyaluronic acid-multifunctional active peptide JM2 sample prepared in step 2 of Example 1, and add 5.6 mg of phenyl-2,4,6-trimethylbenzoyl lithium phosphonate powder , then add 5mL phosphate buffered saline solution to form a mixed solution, stir evenly and set aside;

[0053] Step b. Take 1mL of the mixed solution in step a and add it to the mold, using a power of 25mW / cm 2 UV light was irradiated for 30s to form a hydrogel, which was added to 10 mL of phosphate-buffered saline solution and incubated at 37°C. At 8h, 16h, 24h, 32h, 48h, 72h, 168h, 1 mL of supernatant was collected and 1 mL of phosphate buffered saline was added. The BCA protein quantification kit (BCA Protein Assay Kit) was used to detect the concentration of the polypeptide in samples at different ti...

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PUM

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Abstract

The invention discloses an injectable bioactive hydrogel for inhibiting tumor recurrence after resection and promoting tissue repair, a preparation method and application thereof, and relates to the fields of biomedical materials, tissue engineering and medicine. The injectable bioactive hydrogel contains high molecular material, multifunctional active peptide and water. The polymer material is grafted with the multifunctional active peptide by a grafting agent, and photo-crosslinked and cured by a photoinitiator under ultraviolet light to form the injectable bioactive hydrogel. The injectable bioactive hydrogel of the present invention can continuously and slowly release the multifunctional active peptide grafted on the hydrogel macromolecular material during the whole stage from skin tumor resection to wound repair, and it can be implemented in liquid state and photo-crosslinked solidification, It can fill defects of different shapes and better achieve the combined therapeutic effect of inhibiting tumor recurrence and promoting tissue repair. It is simple to manufacture, easy to use, safe and durable in curative effect, and has clinical application potential.

Description

technical field [0001] The invention relates to the fields of biomedical materials, tissue engineering and medicine, in particular to an injectable bioactive hydrogel for inhibiting tumor recurrence after resection and promoting tissue repair, its preparation method and application. Background technique [0002] Clinical treatments for tumors include surgery, drug therapy, and radiation therapy. For some large and aggressive tumors, surgery is the most traditional treatment method, which can fundamentally inhibit tumor growth and metastasis. Due to incomplete eradication of cancer cells after surgical resection, tumor recurrence remains a major challenge to patient survival. At the same time, it is difficult to repair the wound normally after tumor resection, and even cause long-term difficult-to-heal wounds. [0003] Usually, chemotherapy and radiation are used to prevent the tumor from coming back. Many studies have reported that nanoparticles loaded with small molecule...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61L27/20A61L27/22A61L27/50A61L27/52A61L27/56
CPCA61L27/20A61L27/227A61L27/50A61L27/52A61L27/56A61L2300/252A61L2300/412A61L2300/416A61L2400/06C08L51/02C08L89/00
Inventor 李海燕何丹
Owner SHANGHAI JIAOTONG UNIV
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