New application of DCIK cell culture supernatant preparation

A technology of DCIK and cell culture, applied in the application field of DCIK cell culture supernatant preparation in the preparation of intra-antibody tumor drugs, can solve the problem of unsatisfactory clinical application effect of a single cytokine, and achieve good anti-tumor activity effect

Inactive Publication Date: 2010-03-03
SHANGHAI UNIV
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Problems solved by technology

It is reasonable to speculate that the mixed cytokine network in the cell culture supernatant may also have tumor-specific killing activity; moreover, due to the pleiotropy, antagonism, bidirectionality of cytokine action, and the i

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  • New application of DCIK cell culture supernatant preparation
  • New application of DCIK cell culture supernatant preparation
  • New application of DCIK cell culture supernatant preparation

Examples

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[0013] For the preparation method of DCIK cell culture supernatant preparation, please refer to the Chinese patent named "Supernatant of DCIK cell culture and its application" with application number 200810038429.4.

[0014] The materials used are as follows: The materials and methods are as follows:

[0015] 1. Experimental materials

[0016] 1. Biological agents

[0017] RPMI 1640 medium: purchased from Hyclone;

[0018] Newborn fetal bovine serum: purchased from Hangzhou Sijiqing Company;

[0019] Complete medium: RPMI 1640 medium with 10% newborn fetal calf serum;

[0020] 5-FU specification; 250mg / piece, produced by Shanghai Hualian Pharmaceutical Co., Ltd.

[0021] 2. Reagent formula

[0022] 1×PBS (PH7.4): Dissolve 8.0gNaCl, 0.2gKCl, 1.44gNa2HPO4 and 0.24gKH2PO4 with 800ml high-purity water; adjust the pH of the solution to 7.4 with HCl, and add water to make the volume to 1 liter. Autoclave at 121°C for 20 minutes; store at room temperature.

[0023] Trypsin: 0.25% Trypsin (Invitrog...

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Abstract

The invention relates to the application of a DCIK cell culture supernatant preparation in the preparation of in-vivo anti-tumor medicines. The invention indicates that the DCIK preparation has 58.10percent of the tumor inhibition rate and favorable anti-tumor activity in vivo; and the tumor inhibition rate reaches up to 88.20 percent when the DCIK preparation and 5-fu are combined, which promptsthat the DCIK preparation and the 5-fu have synergistic effect in vivo. A preliminary result proves that the DCIK preparation has favorable in-vivo application effectiveness to a human gastric cancerSGC-7901 naked mouse transplantation tumor model, and the potential development value as a medicine for preventing and controlling human gastric cancer is displayed whatever the preparation is used singly or combined with chemotherapeutics. The invention indicates that the DCIK preparation really has final anti-tumor biological effect under the complex environments in vivo and fully shows the application development values when the DCIK preparation is used singly or combined with the chemotherapeutics.

Description

technical field [0001] The invention relates to a new application of a DCIK cell culture supernatant preparation, in particular to an application of a DCIK cell culture supernatant preparation in the preparation of an intrabody tumor drug. Background technique [0002] In recent years, biological therapy has become a rapidly developing research field in tumor treatment technology, among which cytokine therapy has shown a good development prospect. The anti-tumor mechanism of cytokines mainly involves: controlling the growth of cancer cells and promoting differentiation; regulating the host's immune response; directly killing tumor cells; inducing tumor cell apoptosis and interfering with the growth cycle of tumor cells; destroying tumor blood vessels and nutrient supply ; Stimulate hematopoietic function, promote bone marrow recovery, etc. [0003] DCIK cells are effector cell populations obtained by co-culture of dendritic cells (DC) and cytokine-induced killer cells (CIK)...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61P35/00A61K35/15
Inventor 陈宇光李艳霞石英俊吴涤梵张尚权姚刚李逸平
Owner SHANGHAI UNIV
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