Molecular biology identification method for dry sea cucumbers
A technology of molecular biology and dried sea cucumber, applied in the field of molecular biological identification of dried sea cucumber, can solve the problems of lack of scientific identification technology, inaccurate morphological identification of dried sea cucumber, etc., achieve high yield, reduce costs, and save time Effect
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[0057] The present invention will be described in further detail below in conjunction with specific embodiment: the molecular biology identification method of dried sea cucumber, comprises the following steps:
[0058] (1) The high-salt method is used to extract the dried sea cucumber DNA, and the specific extraction steps are:
[0059] ① Take 100-200 mg of dried sea cucumber, soak it in Milli-Q water for 5-6 hours, and grind it into powder in liquid nitrogen;
[0060]② Transfer to a 50ml centrifuge tube, add 10ml extraction buffer, its composition is 50mM Tris, 100mM NaCl, 150mM EDTA, 1% SDS, 0.30mg / ml proteinase K, 0.1% mercaptoethanol, pH 8.0, digest at 65°C for 3h;
[0061] ③Slowly add 3ml 6mol / L NaCl, salt out to remove excess protein and polysaccharide, centrifuge at 10,000rpm at 4°C for 10 minutes, and collect the supernatant;
[0062] ④ Add phenol, chloroform, and isoamyl alcohol in an equal volume ratio of 25:24:1, centrifuge at 15,000 rpm for 10 minutes, and collect...
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