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High-density array type cell growth cover glass experimental device

An experimental device and an array-type technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of large demand for liquid medicine, cumbersome operation steps, and multiple freezing spaces, and achieve The operation is simple and easy, the freezing space is saved, and the cultivation efficiency is high.

Inactive Publication Date: 2010-03-10
李宏 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. The coverslips are easy to move and overlap on the smooth bottom of the bottle. Some coverslips grow cells on both sides due to gaps caused by overlapping, so that the growth conditions and areas of cells on each coverglass are different.
[0006] There is a problem of deviation in the experimental results due to the poor identity of the slides; in order to avoid this problem, the experimenters usually arrange the coverslips loosely, and as a result, the number of cell slides prepared each time is very limited, generally only 7~ 10 tablets, it is difficult to meet the needs of multi-level and multi-index research;
[0007] 2. There is no special storage device for climbing slices. When the climbing slices are stored at -20°C for backup, it not only takes up a lot of freezing space and causes energy waste, but also takes a lot of trouble to take the climbing slices every time. damage the slide;
[0008] 3. There is no dedicated experimental device for climbing slices, and each experiment is to place the climbing slices directly on the bottom of the vessel, and use the vessel as a container to add a medicinal solution that can submerge the climbing slices. The demand for medicinal liquid is large; at the same time, due to the The surface area is large, and the liquid medicine is easy to evaporate during the experiment, which not only wastes the liquid medicine, but also makes it difficult to obtain accurate experimental results; every time a different liquid medicine is changed, the climbing piece needs to be taken out to dry the upper and lower surfaces, and the experimental operation steps are very cumbersome and inefficient

Method used

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  • High-density array type cell growth cover glass experimental device
  • High-density array type cell growth cover glass experimental device
  • High-density array type cell growth cover glass experimental device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 as figure 1 , 2 Shown:

[0034] There is a circular slide plate 1 which is 2 mm smaller than the diameter of the culture vessel, and the slide plate 1 is made of autoclavable silica gel, metal or other materials. Both sides of the upper surface of the loading plate 1 are provided with handles 4 with a height of 0.5-0.8 to facilitate the taking and placing of the loading plate 1 . On the carrier plate 1, there are ordered downward concave circular grooves 2 arranged in an orderly manner. The depth of the circular grooves 2 is 0.3-0.4mm. The circular grooves 2 are equipped with circular slides that match the bottom of the grooves 2 3. The glass slide 3 is a standard glass slide with a thickness of 0.14-0.17 mm, and the right side wall of the tank 2 is provided with a slice-taking gap 5 . In order to distinguish the front and back of the slides, marks can be made on the slides, such as the letter "R" on the front of the slides with grinding (blasting) sand.

...

Embodiment 2

[0047] Example 2 as image 3 , 4 Shown:

[0048] Others are the same as in Embodiment 1, except that the groove 2 is surrounded by a silica gel plate and the protruding ring 10 on it, and the chip-taking notch 5 is arranged on the protruding ring 10 .

Embodiment 3

[0049] Example 3 as Figure 5 Shown: Others are the same as in Example 1, except that groove 2 has a round shape and a large square shape. In the same experimental system, in addition to obtaining round cell slides, large square slides can also be obtained. For square slides The cells or their lysates are collected in centrifuge tubes according to conventional physical (such as cell scraping) or chemical (such as cell lysing) methods, and then enter the procedure of DNA, RNA or protein sample preparation.

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Abstract

The invention discloses a culture device, a storage device and an experimental device for high-density array type cell growth cover glass. The culture device is provided with a slide plate (1), slotsin ordered arrangement are arranged on the slide plate (1), the depth of each slot (2) is 0.3-0.4mm, and a slide (3) which is matched with the bottom shape of the slot (2) is arranged in the slot (2).The storage device is provided with a bracket (6) which is provided with a plurality of openings (7) which are in ordered arrangement and are used for placing a round growth cover glass, the length of the opening (7) is smaller than the diameter of the round slide (3), the middle width of the opening (7) is bigger than that of two ends, and the width of two ends is 0.15-0.18mm. The experimental device is an experimental plate (8) which is provided with a plurality of grooves (9) which are in ordered arrangement and are used for placing the growth cover glass, the shape of the cross section ofthe groove (9) is matched with the shape of the slide (3), and the depth is 4-6mm. The invention has the advantages of convenient use, high efficiency, favourable identity and the like.

Description

[0001] The present invention is a divisional application with the patent application date of 20060831 and the application number of 200610047607.0, and the name of the invention is "high-density array cell climbing sheet culture device, storage device and experimental device". Technical field: [0002] The invention relates to a cell culture device, in particular to a high-density array type cell culture device, a storage device and an experiment device which are convenient to use, high in efficiency and good in identity. Background technique: [0003] In vitro cell culture simplifies environmental factors, eliminates the influence of some complex interference factors in vivo experiments, and is conducive to the application of various physical, chemical and biological and other external processing factors to explore and reveal the law of cell activities; in vitro cell culture is convenient for the use of various techniques and methods , analyze and observe the morphological a...

Claims

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Application Information

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IPC IPC(8): C12M3/04
Inventor 李宏刘佳孔庆友孙媛文姝陈晓燕
Owner 李宏
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