High-density array type cell growth cover glass experimental device
An experimental device and an array-type technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of large demand for liquid medicine, cumbersome operation steps, and multiple freezing spaces, and achieve The operation is simple and easy, the freezing space is saved, and the cultivation efficiency is high.
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Embodiment 1
[0033] Example 1 as figure 1 , 2 Shown:
[0034] There is a circular slide plate 1 which is 2 mm smaller than the diameter of the culture vessel, and the slide plate 1 is made of autoclavable silica gel, metal or other materials. Both sides of the upper surface of the loading plate 1 are provided with handles 4 with a height of 0.5-0.8 to facilitate the taking and placing of the loading plate 1 . On the carrier plate 1, there are ordered downward concave circular grooves 2 arranged in an orderly manner. The depth of the circular grooves 2 is 0.3-0.4mm. The circular grooves 2 are equipped with circular slides that match the bottom of the grooves 2 3. The glass slide 3 is a standard glass slide with a thickness of 0.14-0.17 mm, and the right side wall of the tank 2 is provided with a slice-taking gap 5 . In order to distinguish the front and back of the slides, marks can be made on the slides, such as the letter "R" on the front of the slides with grinding (blasting) sand.
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Embodiment 2
[0047] Example 2 as image 3 , 4 Shown:
[0048] Others are the same as in Embodiment 1, except that the groove 2 is surrounded by a silica gel plate and the protruding ring 10 on it, and the chip-taking notch 5 is arranged on the protruding ring 10 .
Embodiment 3
[0049] Example 3 as Figure 5 Shown: Others are the same as in Example 1, except that groove 2 has a round shape and a large square shape. In the same experimental system, in addition to obtaining round cell slides, large square slides can also be obtained. For square slides The cells or their lysates are collected in centrifuge tubes according to conventional physical (such as cell scraping) or chemical (such as cell lysing) methods, and then enter the procedure of DNA, RNA or protein sample preparation.
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