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6,11 type human papillomavirus fluorescence quantitative PCR detection method and kit thereof

A technology for human papillomavirus and detection methods, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of reducing the hybridization rate, affecting the detection rate of HPV2 DNA, and saving manpower The effect of material resources, avoiding false negative results, and saving monitoring time

Inactive Publication Date: 2010-03-24
YANGTZE RIVER PHARMA GRP BEIJING HAIYAN PHARMA
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Problems solved by technology

In situ hybridization is the use of probes to hybridize with DNA in tissues. First, the double-stranded DNA in the probes and tissues is denatured into single strands, and then the probes are hybridized with tissues. The key factor affecting in situ hybridization is the hybridization strand. During the hybridization process, part of the probe single-strand anneals, which reduces the hybridization rate and affects the detection rate of HPV2DNA

Method used

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  • 6,11 type human papillomavirus fluorescence quantitative PCR detection method and kit thereof
  • 6,11 type human papillomavirus fluorescence quantitative PCR detection method and kit thereof
  • 6,11 type human papillomavirus fluorescence quantitative PCR detection method and kit thereof

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Embodiment 2

[0055] Embodiment 2---clinical testing

[0056] Using the above method to detect 115 clinical samples, including 42 patients with human papillomavirus 6 and 11, the positive rate of detection is 99%, the accuracy rate is 98.6%, and it can accurately quantify human papillomavirus 6 and 11 analysis, far superior to the enzyme-linked immunosorbent assay (ELISA) method. The invention has the advantages of good specificity, high sensitivity, accurate quantification, rapidity, convenience and high repeatability, and can quickly and quantitatively detect types 6 and 11 human papillomaviruses. In addition, the fluorescent quantitative PCR detection method of human papillomavirus types 6 and 11 reduces the workload and cost of PCR diagnosis of clinical samples, and has potential application value.

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Abstract

The invention relates to a 6,11 type human papillomavirus fluorescence quantitative PCR detection method and kit thereof and PCR amplification primer and probe sequence for 6,11 type nucleotide segments of human papillomavirus. The primer sequence comprises: primer pair of human papillomavirus 6,11 type general upstream primer and general downstream primer, upstream primer complementation sequenceor downstream primer complementation sequence, also comprises primer sequences obtained in range of upstream primer extending towards 5'end by 10 basic groups and the upstream primer extending towards 3'end and 5'end by 10 basic groups. The probe sequence comprises: fluorescent probe: SEQ ID NO:3 and its complementation sequence, and probe sequence obtained in range of the extending SEQ ID NO:3 and its complementation sequence towards 3'end and 5'end by 10 basic groups. The method is used for quantitative detection of 6,11 type human papillomavirus with real clinical application value.

Description

technical field [0001] The invention relates to a fluorescent quantitative PCR detection method for rapidly quantitatively detecting type 6 and type 11 human papillomavirus (Humanpapillomavirus, HPV) serum DNA and a kit thereof, belonging to the field of biotechnology. Background technique [0002] Human papillomavirus (Human papillomavirus, HPV) is an epitheliophilic virus, which is widely distributed in humans and animals and has a high degree of specificity. For a long time, it has been known that HPV can cause benign tumors and warts in humans, such as growth Human warts common on the skin and mucous membranes near the genitals, genital warts, and papillomas growing on the mucous membranes. HPV is a species-specific epitheliophilic virus. The HPV genome is a closed-circle double-stranded DNA with a molecular weight of 5×10 6 dalton. According to function, it can be divided into three regions: early region (E region), late region (L region) and non-coding region (ncr). ...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 郑宜婷唐先兵石和鹏李晓雯肖静
Owner YANGTZE RIVER PHARMA GRP BEIJING HAIYAN PHARMA
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