2 results about "Human papillomavirus types" patented technology

This invention provides a non-classical crRNA, reagent, kit, and application for one-step RPA-Cas12a nucleic acid detection. Based on classic crRNA, the non-classical crRNA shortens the 5' end by two bases and / or adjusts the stem-loop base structure to one of UCUUUU, UCUUU, UGUUU, UGUU, or UAAGU. This non-classical crRNA structure reduces the affinity of the Cas12a-crRNA complex for the target DNA, weakens the cis-cleavage activity of Cas12a, and increases the trans-cleavage activity of Cas12a, without requiring additional base modifications. This invention achieves a detection limit of 1 fM in the detection of Group B Streptococcus and Human Papillomavirus 16, with a sensitivity approximately 10-fold higher than before optimization.

This invention discloses a germplasm screening and identification kit for anthracnose-resistant Camellia oleifera and its application, belonging to the technical field of germplasm screening and identification of anthracnose resistance genes in Camellia oleifera. Based on eight anthracnose resistance genes in Camellia oleifera, this invention develops a germplasm screening and identification kit for anthracnose-resistant Camellia oleifera. This invention enables rapid and efficient identification of anthracnose-resistant Camellia oleifera germplasm and can be used for high-throughput screening of anthracnose-resistant Camellia oleifera lines, providing a new method for disease-resistant breeding of Camellia oleifera.