Preparation method of anti-avian influenza virus, newcastle disease virus, chicken bursa virus specific transfer and antimicrobial peptide

A technology of chicken bursal virus and Newcastle disease virus, which is applied to the preparation method of peptides, specific peptides, chemical instruments and methods, etc., can solve the problems of non-specificity of diseases, unstable treatment effect, etc., and achieve the goal of improving immunity Effect

Inactive Publication Date: 2010-03-31
TIANJIN SHENGJI GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the antibacterial peptides and transfer factors prepared in the prior art that have no specificity for diseases and unstable therapeutic effects, and provide an a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of anti-avian influenza virus, Newcastle disease virus, chicken bursal virus specific transfer factor and antibacterial peptide, steps:

[0018] (1). Immunity:

[0019] Immunize 90-day-old experimental pigs with 1 mL of avian influenza virus vaccine, Newcastle disease virus vaccine, and chicken bursal virus vaccine intramuscularly, and set up a blank control group at the same time. On the 15th day of the first immunization, 2 mL of each of the above vaccines was injected intramuscularly as a booster immunity;

[0020] (2) Closely monitor the immune antibody level of the vaccine in the experimental pig. When the antibody level reaches a peak value, take the spleen of the experimental pig aseptically and prepare a monolayer of lymphocytes with the spleen. The steps are:

[0021] A. Processing pig spleen

[0022] Take fresh pig spleen and place it in a sterile glass container, add PBS (pH 7.2) solution and soak for half an hour (or soak in brogeramin...

Embodiment 2

[0049] A preparation method of anti-avian influenza virus, Newcastle disease virus, chicken bursal virus specific transfer factor and antibacterial peptide, steps:

[0050] (1) Immunity:

[0051] The 50-day-old experimental pigs were immunized with 1.2 mL of avian influenza virus, Newcastle disease virus, and chicken bursal virus vaccines by intramuscular injection, and a blank control group was established simultaneously. On the 7th day after the first immunization, 2.5 mL of each of the above vaccines was injected intramuscularly. Second immunization; on the 10th day after the second immunization, intramuscular injection of 3 mL of the above vaccine was used as the third immunization;

[0052] (2) Closely monitor the immune antibody level of the vaccine in the experimental pig. When the antibody level reaches a peak value, take anticoagulated blood aseptically, and prepare a monolayer of lymphocytes with anticoagulated peripheral blood. The steps are:

[0053] Take 250ml of...

Embodiment 3

[0060] A preparation method of anti-avian influenza virus, Newcastle disease virus, chicken bursal virus-specific transfer factor and antibacterial peptide

[0061] (1) Immunize 40-day-old experimental pigs with intramuscular injection of 5 mL each of avian influenza virus vaccine, Newcastle disease virus vaccine, and chicken bursal virus vaccine. On the 10th day after the first immunization, boost the immunization with 2 mL each of the above vaccines;

[0062] (2) Closely monitor the immune antibody level of the vaccine in the experimental pig. When the antibody level reaches a peak value, aseptically take the spleen or anticoagulant blood of the experimental pig, and prepare a monolayer of lymphocytes with the spleen or anticoagulant blood;

[0063] (3) Subculture the lymphocyte monolayer;

[0064] (4) After the passaged lymphoid cells grow into a single layer, they are induced and cultured with phytohemagglutinin to obtain specific transfer factors and antibacterial peptide...

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Abstract

The invention discloses a preparation method of anti-avian influenza virus, newcastle disease virus, chicken bursa virus specific transfer and antimicrobial peptide, which includes the steps of: (1) using avian influenza viral vaccine, newcastle disease virus vaccine, chicken bursa viral vaccine immune experiment pig; (2) closely monitoring vaccine immune antibody level of experiment pig in vivo,when the antibody reaches a peak value, sterilizedly fetching splenic organ or anticoagulated blood of experiment pig for preparing lymphocyte mono-layer; (3) executing subculture for lymphocyte mono-layer; (4) inducing and culturing the lymph subculture cell after which grows into a mono-layer with phaescolosaxin, then the anti-avian influenza virus, newcastle disease virus chicken bursa virus specific transfer and antimicrobial peptide are obtained. The product prepared by the method does not need to be purified further, and can be treated by ultrasonic and freeze thawing simple process intoa mixer for directly being used for bird in vivo, thereby playing roles for resisting avian influenza virus, newcastle disease virus, chicken bursa virus and bacteria, simultaneously improving immunity of bird.

Description

technical field [0001] The invention relates to a preparation method of antiviral specific transfer factor and antibacterial peptide, in particular to a production method of anti-avian influenza virus, Newcastle disease virus, chicken bursal virus specific transfer factor and antibacterial peptide. Background technique [0002] Transfer factor is a lymphokine extracted from normal animal white blood cells sensitized by foreign antigens, or a lymphokine extracted from convalescent animal blood cells stimulated by certain antigens. The former is called non-specific transfer factor, and the latter is called specific transfer factor. The most important thing about these two kinds of transfer factors is that they can transfer cellular immune function in vivo and in vitro, and as an immune preparation for adoptive immunotherapy, they play a variety of important roles in the immune system. [0003] The current preparation method of transfer factor is: remove fat and connective tis...

Claims

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Application Information

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IPC IPC(8): C07K1/00C07K14/52C07K14/47
Inventor 田杏芳王连民
Owner TIANJIN SHENGJI GRP CO LTD
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