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Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same

A technology of galacto-oligosaccharides and Aspergillus oryzae, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems that the disaccharide component lactose is not well separated and galactose cannot be removed , to achieve the effect of easy industrial production, short cycle and simple process

Active Publication Date: 2012-05-09
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent removes some monosaccharide components well, but galactose cannot be removed, and the disaccharide component lactose is not well separated

Method used

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  • Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same
  • Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same
  • Aspergillus oryzae and method for preparing high purity galacto-oligosaccharides by using same

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Strain BLB-21 was inoculated with the seed solution with a glucose concentration of 300g / L for expansion. The secondary seed solution (concentration: 0.52% of the wet weight of mycelium) was added to 20% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 25-30° C. for 40 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cations-weak basic anions-strong acidic cation exchange resins were used for separation and desalination at a temperature of 30-35°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 55.80% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0040] The above sugar solution is separated by cellulose acetate nanofiltration membrane under the conditions of 2.0-2.4MPa pressure, 20-25% fee...

Embodiment 2

[0044] Strain BLB-21 was inoculated with the seed solution with a glucose concentration of 400g / L for expansion. The secondary seed solution (concentration: 0.45% of the wet weight of mycelium) was added to 30% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 30-35° C. for 30 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cation-weak basic anion-strong acidic cation exchange resin was used for separation and desalination at a temperature of 35-40°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 56.35% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0045] The above sugar solution is separated under the conditions of 2.4-2.7MPa pressure, 20-25% feed concentration, 4BV of water, and 25-30°C temper...

Embodiment 3

[0049] The strain BLB-21 was inoculated with the seed solution with a glucose concentration of 500g / L for expansion. The secondary seed solution (concentration: 0.56% of the wet weight of mycelia) was added to 40% lactose solution in an amount of 10% (V / V) for fermentation, adjusted to pH 5.5, and fermented at 35-40° C. for 20 hours. The mixture of sugar components was preliminarily obtained by ultrafiltration. After decolorization by activated carbon, strong acidic cations-weak basic anions-strong acidic cation exchange resins were used for separation and desalination at a temperature of 45-50°C, an injection volume of 10L / h, and a resin height of 1m. A sugar solution with a galacto-oligosaccharide content of 55.72% was detected by HPLC. At this time, the sugar components are glucose, galactose, lactose, and galactooligosaccharides.

[0050] The above sugar solution is separated by cellulose acetate nanofiltration membrane under the conditions of 2.7-3.0 MPa pressure, 25-30%...

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Abstract

The invention relates to a method for preparing high purity galacto-oligosaccharides, comprising the following product separation and purification steps: Aspergillus oryzae fermentation, ceramic membrane ultrafiltration, nanofiltration separation and the like. In the method, the Aspergillus oryzae separated from the soil is adopted as an original strain and the Aspergillus oryzae BLB-21 (with preservation number of CGMCC No.2951), a high efficiency transformed strain obtained through mutation screening in the laboratory, is directly used to ferment high concentration lactose solution, thus avoiding the steps of enzyme purification and the like in the process of preparing the galacto-oligosaccharides by an enzyme method and saving time and labor. The high purity galacto-oligosaccharides are obtained by ultrafiltration and nanofiltration separation, the process is ideal, the conditions are mild and the method has extensive industrial production prospect.

Description

technical field [0001] The invention belongs to the field of functional oligosaccharide production, and mainly relates to a production method of aspergillus oryzae directly fermenting a high-concentration lactose solution to carry out galacto-oligosaccharides. Background technique [0002] Galacto-oligosaccharides (GOS) are non-digestible oligosaccharides. Compared with other non-digestible oligosaccharides, they can specifically promote the proliferation of bifidobacteria in the intestinal tract. The raw materials for production have a wide range of sources and are safe to use. High stability, stable to heat treatment of food processing and so on. Therefore, the production and application of galactooligosaccharides has great potential in the field of food industry. At present, the manufacturers of galacto-oligosaccharides in the world are mainly in Japan. The production of galacto-oligosaccharides in my country has not yet been scaled up and is in the research stage. Gala...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/18C12R1/69
Inventor 刘宗利王乃强袁卫涛栾庆民冯志臣滕慧李庆华李双茹
Owner BAOLINGBAO BIOLOGY
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