New process for fermenting Beta-polymalic acid

A technology of polymalic acid and a new process, applied in the field of fermentation engineering, can solve the problems of pH fluctuation, long fermentation period, slow growth of bacteria, avoid the difficulty of dissolved oxygen and mass transfer, solve the slow growth of bacteria, and promote the growth of bacteria. production effect

Inactive Publication Date: 2010-04-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem solved by the present invention is: adding alkaline solution to accurately control the pH of the fermentation broth, solving the problems of pH fluctuations caused by adjusting the pH with calcium carbonate, difficulties in dissolved oxygen mass transfer, and Ca ions easily reacting with other culture medium components to cause precipitation ;Use organic nitrogen source and inorganic nitrogen source in combination to solve the problems of slow bacterial growth and long fermentation cycle; adjust tank pressure, ventilation and stirring speed to control the concentration of dissolved oxygen in the fermentation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Produce β-polymalic acid by fermentation as follows:

[0027] The first step, culture medium preparation

[0028] ①Activation medium: potato dextrose medium, sterilized at 121°C for 15 minutes;

[0029] ② Preparation of seed medium (w / v): 8% glucose, 0.2% NaNO 3 , 0.01% KH 2 PO 4 , 0.02% MgSO 4 ·7H 2 O, 0.05% KCl, 1g / l peptone, pH 4.0-4.5, sterilized at 121°C for 15min.

[0030] ③ Preparation of fermentation medium (w / v): 8-15% glucose, 0.2% NaNO 3 , 0.01% KH 2 PO 4 , 0.02% MgSO 4 ·7H 2 O, 0.05% KCl, sterilized at 121°C for 15 minutes.

[0031] The second step, the activation of bacteria

[0032] The strain of Aureobasidium pullulans ipe-1 stored at 4°C was transferred to the slant of the activation medium, and the activation culture was carried out at 25°C for 72 hours.

[0033] The third step, seed cultivation

[0034] Inoculate the activated seed culture on the slant into a 500ml Erlenmeyer flask containing 100ml of fermentation medium, and culture it w...

Embodiment 2

[0038]Produce β-polymalic acid by fermentation as follows:

[0039] The first step, culture medium preparation

[0040] Same as Example 1

[0041] The second step, the activation of bacteria

[0042] Same as Example 1

[0043] The third step, seed cultivation

[0044] Same as Example 1

[0045] The fourth step, inoculation and fermentation

[0046] The culture obtained in the third step was inoculated into a 7L fermenter with 4L fermentation medium at an inoculum size of 10%. sky. Finally, the content of β-polymalic acid in the fermentation liquid was 7.50 g / L, and the dry weight of the bacteria was 8.38 g / l.

Embodiment 3

[0048] Produce β-polymalic acid by fermentation as follows:

[0049] The first step, culture medium preparation

[0050] Same as Example 1

[0051] The second step, the activation of bacteria

[0052] Same as Example 1

[0053] The third step, seed cultivation

[0054] Same as Example 1

[0055] The fourth step, inoculation and fermentation

[0056] The culture obtained in the third step was inoculated into a 7L fermenter containing 4L fermentation medium with a 10% inoculum size, pH 7.0, temperature 25°C, rotation speed 600r / min, 0.75vvm, and cultivated for 7 days. Finally, the content of β-polymalic acid in the fermentation broth was 2.64g / L, and the dry weight of the bacteria was 7.08g / l.

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PUM

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Abstract

The invention discloses a new process for fermenting a Beta-polymalic acid by using a microorganism fermentation process, which is realized in such a way: culturing Aureobasidium pullulans ipe-1 in a culture medium containing carbon sources, nitrogen sources, inorganic salts sand trace elements under the condition of controlling the pH value and dissolved oxygen; controlling the fermentation pH value by aqueous alkali; and adjusting the ventilation quantity, the tank pressure and the stirring speed to control the concentration of the dissolved oxygen. The pH value is 5.5-7.0, an organic carbon source and an inorganic source are matched for use, the concentration of the dissolved oxygen is controlled to be larger than 70 percent within 16h before fermentation, and the concentration of the dissolved oxygen is controlled to be 10-80 percent after fermentation, thereby being suitable for accumulating Beta-polymalic acid in a large quantity.

Description

technical field [0001] The technical scheme of the invention belongs to the technical field of fermentation engineering, and specifically relates to a new fermentation process that uses alkaline solution to control fermentation pH, and adjusts tank pressure, ventilation rate and stirring rate to control dissolved oxygen concentration. Background technique [0002] In 1969, Shimada and Matsushima, the microbiologists studying Penicillium cyclopium, first discovered polymalic acid in the culture medium of the fungus. In 1979, Vert et al first synthesized a water-soluble aliphatic polyester - polymalic acid, which uses polymalic acid as the only monomer. In 1989, Fisher et al. isolated polymalic acid from Physarum Polycephalum cells and found that it was an inhibitor of DNA polymerase α. So far, foreign scholars represented by Vert, Cammas, Kajiyama, etc. have done in-depth research on the properties and applications of polymalic acid. [0003] Polymalic acid is a special kin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/645
Inventor 万印华曹伟锋苏仪沈飞陈向荣伊守亮赵方
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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