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Smilax china effective fractions and extraction as well as purification process thereof

A technology for extracting effective parts and groups, which is applied in the field of extraction and purification of effective parts and groups of Smilax smilax and its extract products. and other issues, to achieve the effect of being suitable for large-scale production, controllable quality standards, and controlling environmental pollution

Inactive Publication Date: 2012-03-28
湖北福人药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water extraction and alcohol precipitation method belongs to the traditional extraction process, the effective substance basis of the extract is not clear, the quality standard is difficult to control, and the clinical dosage is too large; the ethanol extraction-macroporous resin purification method uses ethanol as the extraction solvent, and there are extraction and purification The process is cumbersome, the production cost is high, the environmental pollution is serious, and the safety hazard is relatively large, which does not meet the modernization requirements of the traditional Chinese medicine industry.
The present invention uses water as the extraction solvent, and the extract is purified by macroporous adsorption resin to obtain a relatively high content of effective fractions of flavonoids, saponins and tannins. This extraction and purification technology has no relevant data reports at home and abroad.

Method used

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  • Smilax china effective fractions and extraction as well as purification process thereof
  • Smilax china effective fractions and extraction as well as purification process thereof
  • Smilax china effective fractions and extraction as well as purification process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Extraction process test of effective parts of Smilax smilax with water as solvent

[0043] (1) Water reflux extraction process test: using L 9 (3 4 ) orthogonal test method, taking the total flavonoids, total saponins and total tannins of sarsaparilla as investigation indicators, determine the process parameters of factors such as soaking time, water addition (multiple of smilax medicinal material amount) and extraction time-number of times, and use ultraviolet - The content of total flavonoids, total saponins and total tannins in the extract was determined by visible spectrophotometry, and the content of total flavonoids, total saponins and total tannins and the yield of dry paste were used as evaluation indicators for comparative analysis. The results are shown in Table 1-Table 7.

[0044] Table 1 Test factor level table

[0045]

[0046] Table 2 Orthogonal experiment design and result analysis

[0047]

[0048]

[0049]

[0050] Among them: we...

Embodiment 2

[0072] Example 2 Macroporous resin purification process test of effective parts of Smilax smilax

[0073] The sarsaparilla extract that extracts with aforementioned optimal extraction condition is carried out following test:

[0074] (1) Screening test of macroporous resin

[0075] Resin source: D101 resin (Tianjin Haiguang Chemical Co., Ltd.), AB-8, HPD100 resin (Hebei Baocang Co., Ltd.).

[0076] ① Static saturated adsorption and desorption elution test of different macroporous adsorption resins on total flavonoids and total saponins in sample solution

[0077] Accurately weigh 1g of the treated macroporous adsorption resin (suction filter until no water drips), put it in a 100mL ground-mouthed Erlenmeyer flask, add 50mL of the test product solution precisely, and place it on a shaker for 24 hours to make it fully After adsorption, the upper layer was taken to determine the concentration of total flavonoids and total saponins respectively. And calculate the saturated adso...

Embodiment 3

[0138] Example 3 Pilot scale-up test

[0139] Take 12Kg sarsaparilla decoction pieces, add 10 times the amount of water to soak for 4 hours, heat and reflux extraction for 3 times, the first and second time are 2 hours each, and the third time is 1 hour, combine the filtrate, concentrate until the volume of the liquid medicine is 10 times the volume of the medicinal material, The sample drug solution was obtained by suction filtration.

[0140] Soak 20Kg of pharmaceutical-grade D101 macroporous resin with an appropriate amount of ethanol, wet-pack the column, and prepare it for later use.

[0141] Adsorption was carried out at a flow rate of 2Bv / h, and the resin bed diameter-to-height ratio was 1:8. After the adsorption was completed, use distilled water 3 times the amount of resin to elute at a flow rate of 2Bv / h until the Molish reaction was negative, and then use 3 70% ethanol twice the volume of the resin column, eluted at a flow rate of 2Bv / h, and collected the eluate. ...

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Abstract

The invention relates to medicinal plant smilax china effective fractions and an extraction as well as purification process, in particular to a method for extracting and purifying the smilax china effective fractions and extract products thereof. The extraction method comprises the following steps of: a. extracting the smilax china by water, condensing an extracting solution and filtrating; b. absorbing filtrate by using a macroporous absorption resin, eluting with water firstly and then eluting with alcohol; and c. collecting alcohol eluting solution, recovering the alcohol, condensing, drying to obtain the extract product.

Description

technical field [0001] The invention relates to a method for extracting and purifying effective parts of medicinal plants, in particular to a method for extracting and purifying effective parts of Chinese medicine sarsaparilla and extract products thereof. Background technique [0002] Smilax is the dry rhizome of Smilax China L., a plant of the family Liliaceae, also known as King Kongteng. It is a traditional Chinese medicine. It is recorded in the 2005 edition of the "Chinese Pharmacopoeia". It is used for soreness of muscles and bones, dripping urine, profuse leukorrhagia, boils and carbuncles. In addition to treating gynecological inflammatory diseases, traditional Chinese medicine is also used to treat tumors, and has achieved good results. At present, there are many Chinese medicine companies in China. Traditional Chinese medicine preparations made from sarsaparilla medicinal materials mainly include Jingangteng capsules, Jingangteng syrup, Jingangteng granules, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P35/00A61K36/896A61K125/00A61P15/00
Inventor 刘焱文陈树和冯芸尹玲黄显章
Owner 湖北福人药业股份有限公司
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