Method for detecting activity of fluorescein diacetate in bottom sediment
A technology of fluorescein diacetate and fluorescein, which is applied in the preparation of test samples, biochemical equipment and methods, and the measurement/inspection of microorganisms. and other issues to achieve a highly targeted effect
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specific Embodiment 1
[0065] The sample used in this example is the bottom mud taken from a certain river, and the sample was collected in a sterilized sample bag with a bottom mud sampler, and after being brought back to the laboratory, it was stored at 4°C for one week and the FDA activity of the sample was determined by the above method.
[0066] The specific FDA activity determination steps of sediment samples are as follows:
[0067] 1) Weigh 0.5-1.0g sample (record the accurate mass of sediment sample) into a 100mL Erlenmeyer flask, add 15mL phosphate buffer (pH=7.6), add an appropriate amount of glass beads, shake and mix; add 0.2mL 2mg / Start the reaction with mL FDA solution, and incubate at 30°C for 30 min with constant temperature and shaking.
[0068] 2) Immediately add 8 mL of chloroform / methanol (2:1 v / v) mixed terminator to terminate the reaction, and shake fully for 5 min.
[0069] 3) Transfer the mixed solution to a 10mL centrifuge tube, centrifuge at 2000r / min for 5min, take the ...
specific Embodiment 2
[0093] The sample used in this embodiment is a bottom mud sample taken from a certain river course, and its sampling and preservation methods are the same as in Example 1.
[0094] The specific FDA activity determination steps of sediment samples are as follows:
[0095] 1) Weigh 0.5-1.0g sample (record the accurate mass of sediment sample) into a 100mL Erlenmeyer flask, add 15mL phosphate buffer (pH=7.6), add appropriate amount of glass beads, shake and mix; add 0.2mL 2mg / Start the reaction with mL FDA solution, and incubate at 30°C for 30 min with constant temperature and shaking.
[0096] 2) Immediately add 8 mL of chloroform / methanol (2:1 v / v) mixed terminator to terminate the reaction, and shake fully for 5 min.
[0097] 3) Transfer the mixed solution to a 10mL centrifuge tube, centrifuge at 2000r / min for 5min, take the supernatant and measure the absorbance at 490nm.
[0098] 4) The drawing of the fluorescein standard curve is the same as in Example 1.
[0099] 5) Co...
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