Bioreactor

A bioreactor and assembly technology, applied in biochemical instruments, biochemical equipment and methods, tissue cell/virus culture devices, etc., can solve problems such as uneven distribution of cells, dead shells, insufficient oxygen supply, etc., to achieve The effect of improving mass transfer efficiency

Active Publication Date: 2010-05-19
INST OF ELECTRICAL ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the prior art, and propose a bioreactor for artificial liver support in vitro and cell culture research, w

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Embodiment Construction

[0046] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0047] The invention consists of a reciprocating rotating cell culture tank assembly module, a gas dispersion control assembly module and a liquid flow direction control assembly module connected in series through a cell culture liquid circulation pipeline. The connections between them are as figure 1 shown. The pipeline from the peripheral equipment of the bioreactor of the present invention is connected to the first port of the tube lumen of the gas dispersion control assembly module (102); the second port of the tube lumen of the gas dispersion control assembly module (102) is connected to the liquid flow direction control The first input port (106) of the assembly module; the output port of the liquid flow control assembly module (103) is connected to the input port of the reciprocating and rotating cell culture tank assembly module (104);...

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Abstract

The invention relates to a bioreactor, which is formed by serially connecting a reciprocating-rotation cell culture tank assembly module (104), a gas-dispersion control assembly module (102) and a liquid flow direction control assembly module (103) through a cell culture solution circulation pipeline. A carrier suspension conglutinated with cells is injected into a cell culture tank of a reciprocating bioreactor, and along with the rotation of a step motor and a double-headed peristaltic pump in the liquid flow direction control assembly module, the output of a preceding state hemodialysis unit or a culture solution in a culture solution container enters the circulation pipeline. When the culture solution reaches a dialysis column (301), a corresponding gas is diffused into the culture solution. Then, the culture solution with the corresponding gas is sent into the cell culture tank. After the exchange of substance with cells in the culture tank, the culture solution containing metabolites of cultured cells is sent back into the preceding state hemodialysis unit or the culture solution container through an output port of the cell culture tank and the culture solution circulation pipeline.

Description

technical field [0001] The invention relates to a medical device, in particular to a bioreactor. Background technique [0002] There are four main types of existing bioreactors, namely: airlift cell culture bioreactors, hollow fiber tube bioreactors, aeration stirring bioreactors and non-bubble stirring bioreactors. Most of them are used for fermentation or plant cell culture and do not meet the requirements of animal cell culture. Since animal cells do not have cell walls, they are sensitive to shear force. Fluid shear and air bubbles in the bioreactor due to stirring and ventilation will inevitably lead to cell damage, even breakage, and death, resulting in failure of the culture process. Bioreactors used for animal cell culture must ensure good mass transfer mixing and minimize the shear force of the fluid to reduce damage to cells. [0003] At present, there is still no dedicated bioreactor device in the field of artificial liver extracorporeal support, and the common ...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 霍小林吴昌哲李明
Owner INST OF ELECTRICAL ENG CHINESE ACAD OF SCI
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