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Optical immunity detecting method based on porous silicon three-element structure microcavity

An immunoassay method and porous silicon technology, applied in optics, nonlinear optics, measuring devices, etc., can solve problems such as the difficulty in repeatedly preparing porous silicon photonic crystals and the deviation of the actual scale of the porous silicon photonic bandgap structure, and meet high Flux detection, reduction of the influence of follow-up factors, and the effect of large surface area

Inactive Publication Date: 2010-05-19
XINJIANG UNIVERSITY
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  • Application Information

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Problems solved by technology

In addition, porous silicon Bragg reflectors, porous silicon microcavity structures, or various porous silicon multilayers based on the photoluminescent properties of porous silicon are mainly used at present through electrochemical corrosion of high and low currents. Due to the influence of many external random factors, the actual scale of the porous silicon photonic bandgap structure will deviate from the actual scale, which will produce a certain disorder, and it is difficult to repeatedly prepare ideal porous silicon photonic crystals in large batches.

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  • Optical immunity detecting method based on porous silicon three-element structure microcavity
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Experimental program
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Embodiment 1

[0024] Embodiment one. Detection of target antigen-bovine serum albumin (BSA) concentration using porous silicon ternary structure microcavity:

[0025] 1. The method of chemical bond coupling is used to immobilize the BSA antibody into the pores of the porous silicon microcavity. The pretreatment steps are as follows:

[0026] 1) Place the porous silicon in an environment at 900°C for 15 minutes to oxidize;

[0027] 2) Soak in 4% APTES diluted with toluene solution for one hour to carry out silanization reaction;

[0028] 3) Rinse repeatedly with toluene, methanol-toluene, methanol, and deionized water, dry in N2 environment, and then bake the sample at 100°C for 10 minutes;

[0029] 4) in 2% glutaraldehyde aqueous solution for 30 minutes, and repeatedly washed with PBS (phosphate buffer solution pH=7.4) to remove residual glutaraldehyde;

[0030] The treated porous silicon microcavities were divided into two groups, one group was immersed in BSA-positive serum, and the ot...

Embodiment 2

[0032] Embodiment two. Detection of human papillomavirus (HPV) L1 antigen and steppe rabbit-tailed mouse egg zona pellucida 3 gene (LZP3) antigen in solution using encoded porous silicon microchambers:

[0033] 1. The porous silicon microcavity of the two structures is pretreated by functionalization using the pretreatment method of the porous silicon microcavity in Example 1. The porous silicon microcavities of the two structures were respectively immobilized with HPV L1 antibody and LZP3 antibody, and the other porous silicon microcavity was immobilized with BSA as a control. Wash with PBST (a mixed solution of 0.1% Tween-20 and phosphoric acid) repeatedly, and block with 3% BSA. The defect transmission peak wavelengths of the three porous silicon microcavities were measured at 1500nm, 3500nm, and 5500nm, respectively, and coded according to the defect transmission peak wavelengths.

[0034] 2. Soak the three kinds of porous silicon microcavities put together in the mixed ...

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Abstract

The invention relates to an optical immunity detecting method based on a porous silicon three-element structure microcavity, which belongs to the technical field of biological medicines, food security and environmental monitoring. An upper Bragg structure and a lower Bragg structure in a porous silicon microcavity adopted by the method are respectively formed by alternately carrying out electrochemical corrosion through three current densities; a probe molecule is fixed in a porous silicon hole at first; then the concentration of a target molecule is detected by the light spectrum peak position change before and after biological reaction; and meanwhile, a carrier is coded by a reflecting light spectrum or a photoluminescence light spectrum of the porous silicon microcavity prepared under different corrosion conditions to realize the identification to an antigen or antibody variety. The optical immunity detecting method has a plurality of excellent properties of a porous silicon and photonic bandgap structure sensor and good structural stability and can realize multielement detection by a coding detection technology. In addition, because the adopted preparation method is simpler and has relatively low cost, the invention has certain commercial application prospects.

Description

technical field [0001] The invention relates to a label-free optical immunological detection method using a porous silicon ternary structure microcavity as a solid-phase sensor carrier, and belongs to the technical fields of biomedicine, food safety and environmental monitoring. Background technique [0002] At present, the research on immunoassay detection technology is very active in the fields of biomedicine, food safety, environmental monitoring, and hormone determination. This technology is mainly based on the specific binding principle between antigen and antibody and combined with modern testing methods. The change of the substrate signal after the specific reaction of the needle molecule is used for high-sensitivity analysis and detection of the target molecule. Among them, the technology of immunoassay using optical signal is optical immunoassay technology. [0003] Immunoassay techniques mainly include two categories: labeling and labeling-free. During immunolabe...

Claims

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Application Information

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IPC IPC(8): G01N33/551G01N33/68G01N33/569G02F1/01
Inventor 吕小毅莫家庆贾振红钟福如李锐
Owner XINJIANG UNIVERSITY