Method for detecting hydroxyl radicals

A determination method and free radical technology, which are used in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of expensive instruments and insufficient sensitivity, and achieve simple and easy operation. Simple and fast, low-cost detection effect

Inactive Publication Date: 2010-06-02
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods or instruments are expensive or not sensitive enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Drawing of standard curve

[0033] Add 2.0×10 -4 mol L -1 9.0 mL of night blue aqueous solution, 5.0 mL of phosphoric acid-sodium dihydrogen phosphate buffer solution with a pH of 3.1 to 3.3, 1.0×10 -3 mol L -1 FeSO 4 Aqueous solution 0.3mL;

[0034] Then add the now-prepared 5.0×10 -4 mol L -1 Add 0.2mL hydrogen peroxide to No. ①, add 0.4mL hydrogen peroxide to No. ②, add 0.6mL hydrogen peroxide to No. ③, add 0.8mL hydrogen peroxide to No. ④, add 1.0mL hydrogen peroxide to No. ⑤;

[0035] No. ⑥ does not add hydrogen peroxide, as a reference solution;

[0036] Mix ① to ⑥ thoroughly, let stand for 15 minutes, dilute with distilled water, and shake well;

[0037] Use a 1cm cuvette and use No. ⑥ as a reference to measure the absorbance of the solutions in the 5 volumetric flasks at 618nm from ① to ⑤;

[0038] Draw a standard curve with the absorbance as the ordinate and the concentration of hydrogen peroxide as the abscissa;

[0039] (2) Obtaining the regress...

Embodiment 2

[0044](1) Drawing of standard curve

[0045] With embodiment 1;

[0046] (2) Obtaining the regression equation

[0047] Same as Example 1

[0048] (3) Determination of hydroxyl radicals

[0049] Add emulsifier 2.0×10 to the 50mL volumetric flask -4 mol L -1 9.0 mL of night blue aqueous solution, 5.0 mL of phosphoric acid-sodium dihydrogen phosphate buffer solution with a pH of 3.1 to 3.3, 1.0×10 -3 mol L -1 FeSO 4 Aqueous solution 0.3mL, the prepared concentration is 5.0×10 -4 mol L -1 0.7mL of hydrogen peroxide, mix well, after standing for 15min, make up volume with distilled water, shake well, use 1cm cuvette, use No. ⑥ in step (1) as reference, measure the absorbance of the solution at 618nm to be 0.252, According to the regression equation calculation of step (2), the concentration of hydroxyl radical is 0.119mg / L;

Embodiment 3

[0051] (1) Drawing of standard curve

[0052] With embodiment 1;

[0053] (2) Obtaining the regression equation

[0054] Same as Example 1

[0055] (3) Determination of hydroxyl radicals

[0056] Add emulsifier 2.0×10 to the 50mL volumetric flask -4 mol L -1 Night blue (C.I 44085) aqueous solution 9.0mL, pH 3.1-3.3 phosphoric acid-sodium dihydrogen phosphate buffer solution 5.0mL, 1.0×10 -3 mol L -1 FeSO 4 Aqueous solution 0.3mL, the prepared concentration is 5.0×10 -4 mol L -1 1.1mL of hydrogen peroxide, mix thoroughly, after standing for 15min, make up volume with distilled water, shake well, use 1cm cuvette, use the ⑥ number in step (1) as reference, measure the absorbance of the solution at 618nm to be 0.401, Calculate the concentration of hydroxyl radicals according to the regression equation of step (2) to be 0.379mg / L.

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Abstract

The invention provides a method for detecting hydroxyl radicals. The method comprises the following steps of: in an aqueous solution, oxidizing night blue by hydroxyl radicals so as to ensure that the night blue fades; selectively absorbing visible light with a wavelength of 618nm; and obtaining the concentration of the hydroxyl radicals in the solution through measuring the absorbancy of the solution. The method can detect the hydroxyl radicals within a concentration range of 0.039-0.379mg / L, has the advantages of simple instrument, low detecting cost, simple and quick operation, high sensitivity, and the like; and metered by the concentration of hydrogen peroxide, the molar absorption coefficient is up to 3.6*104L.mol-1.cm-1.

Description

technical field [0001] The invention relates to a method for measuring hydroxyl radicals. Specifically, it relates to a new method of using a naphthyl diphenylmethane dye-night blue as a chromogenic agent and using a spectrophotometric method to measure hydroxyl radicals. Background technique [0002] Hydroxyl radicals (·OH) are highly toxic and harmful to organisms, and have high reactivity, short lifespan, and low concentration. At present, the methods reported at home and abroad for the determination of hydroxyl radicals include: electron spin resonance method, high performance liquid chromatography, fluorescence method, resonance scattering spectroscopy and spectrophotometry. These methods or instruments are expensive, or the sensitivity is not enough. Contents of the invention [0003] The purpose of the present invention is to overcome the above-mentioned technical disadvantages and provide a method for measuring hydroxyl radicals with the advantages of simple equi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 闻海峰秦琴施文健路荣春杨琴淋周艳
Owner UNIV OF SHANGHAI FOR SCI & TECH
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