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Hircine primary atypical pneumonia combined inactivated vaccine and preparation method thereof

A dual inactivated vaccine and mycoplasma pneumonia technology, applied in the direction of bacterial antigen components, respiratory diseases, bacteria, etc., can solve the problems of indistinguishable diseases, lack of cross protection, mixed infection, etc.

Active Publication Date: 2013-01-30
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Mycoplasma goat pneumonia pathogen that is prevalent in my country lacks cross-protection between Mycoplasma goat subspecies and Mycoplasma ovis pneumoniae, and the resulting diseases are difficult to distinguish clinically, and often cause mixed infections, so it is difficult to adapt to the vaccine made by a single pathogen The actual production in our country, and currently there is no dual inactivated vaccine that can prevent these two pathogens at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1 Prepare culture medium

[0031] From March 11th to 12th, 2005, 42000ml of improved KM2 medium was prepared. 14700ml of 1.7% hydrolyzed milk protein Hank's buffer, 840ml of 25% yeast infusion, 420ml of 1% thallium acetate solution and 420ml of 0.4% phenol red, mixed and sterilized by 10 pounds for 20 minutes, after cooling, add 0.22μm microporous The minimum element medium MEM2 1000ml, healthy horse serum 4200ml, and 20,000 IU penicillin 420ml were sterilized by membrane filtration, the pH value was adjusted to 7.8 with 1mol / L sodium hydroxide solution, and stored at 2°C for later use.

[0032] 2 Mycoplasma proliferation

[0033] 2.1 Proliferation of Mycoplasma: On March 12, 2005, Mycoplasma goat subspecies M87-1 was inoculated with 5ml of improved KM2 medium. After 3 days of cultivation, the pH value of the culture dropped to 7.0. 5% for 3 consecutive expansion cultures, when the pH value of the culture dropped to 7.0, harvest 18000ml of mycoplasma goat subsp. 7 cc...

Embodiment 2

[0050] 1 Prepare culture medium

[0051] From April 1st to 2nd, 2005, 42000ml of improved KM2 medium was prepared. 14700ml of 1.7% hydrolyzed milk protein Hank's buffer, 840ml of 25% yeast infusion, 420ml of 1% thallium acetate solution, 420ml of 0.4% phenol red, mixed and sterilized by 10 pounds for 20 minutes, cooled and then added with a 0.22μm microporous Membrane filtration sterilized minimum element medium (MEM) 21000ml, healthy horse serum 4200ml, 20,000 IU penicillin 420ml, adjusted the pH value to 7.4 with 1mol / L sodium hydroxide solution, and stored at 8°C for later use.

[0052] 2 Mycoplasma proliferation

[0053] 2.1 Proliferation of Mycoplasma: On April 10, 2005, Mycoplasma goat subspecies M87-1 was inoculated with 10 ml of the improved KM2 medium, and after 5 days of cultivation, the pH value of the culture dropped to 6.8. Carry out 3 times of continuous expansion cultures by 5% of the total amount of the culture medium, when the pH value of the culture drops t...

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Abstract

The invention relates to a hircine primary atypical pneumonia combined inactivated vaccine and a preparation method thereof. The preparation method comprises the following steps of: a. a culture medium for vaccine preparation and a preparation method thereof: using an improved KM2 culture medium as the culture medium for vaccine preparation; b. a procedure for propagating mycoplasma; c. a procedure for inactivating the mycoplasma; and d. a vaccine preparation procedure: using the one-step emulsion process to prepare bi-phase oil emulsion vaccine; the liquid-phase composition: accounting for 45-50% of the total amount of the vaccine, and adding 1% Merthiolate solution which accounts for 1% of the total amount of antigens after mixing protonema mycoplasma hircine subspecies and ovine mycoplasma pneumoniae inactivated antigens in a ratio of 1:1; the oil-phase composition: accounting for 50-55% of the total amount of the vaccine and containing ISA206 oil adjuvant; and emulsifying into a water / oil / water (w / o / w) bi-phase oil emulsion inactivated vaccine with slight viscosity. The invention first combines the two dominant pathogens to prepare the combined vaccine, and can simultaneously prevent the two pathogens, thereby avoiding the defect that the single-pathogen preparation vaccine can be only used for one pathogen but not for the other, and being more applicable to the practical production in China.

Description

technical field [0001] The invention relates to the field of mycoplasma pneumonia vaccines, in particular to a dual inactivated vaccine of mycoplasma pneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma goat pneumonia refers to a contact respiratory infectious disease caused by mycoplasma in goats. Its clinical characteristics are high fever, cough, serous and fibrinous inflammation of the chest and pleura, and the acute and chronic course is taken. 22-30%, some as high as 60-80%, and the mortality rate is 15-30%. The disease is common in sheep-raising countries and regions in the world, and seriously affects the development of the world's sheep-raising industry. In my country, this disease has been prevalent for a long time, and the earliest written records can be traced back to 1922. Especially with the rapid development of my country's sheep industry in recent years, the epidemic area of ​​the disease has increased. So far, Gansu, Ningxia...

Claims

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Application Information

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IPC IPC(8): A61K39/116A61K39/02A61P31/04A61P11/00C12N1/20
Inventor 逯忠新储岳峰赵萍高鹏程贺英
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI