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Combined inactivated vaccine for goat mycoplasma pneumonia and preparation method of combined inactivated vaccine

A dual inactivated vaccine, mycoplasma pneumonia technology, applied in biochemical equipment and methods, vaccines, multivalent vaccines, etc., can solve problems such as indistinguishable diseases, mixed infection, etc., and achieve the effect of reducing production costs and preventing infection

Pending Publication Date: 2021-06-18
GUANGXI VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of cross-protection between Mycoplasma capricosum subspecies (MMC) and Mycoplasma ovis pneumoniae (MO), the pathogens of mycoplasma pneumoniae that are prevalent in my country, the resulting diseases are difficult to distinguish clinically and often cause mixed infections, so a single pathogen should be used The vaccine made is difficult to adapt to the actual production in my country, and currently there is no dual inactivated vaccine that can prevent these two pathogens at the same time

Method used

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  • Combined inactivated vaccine for goat mycoplasma pneumonia and preparation method of combined inactivated vaccine
  • Combined inactivated vaccine for goat mycoplasma pneumonia and preparation method of combined inactivated vaccine
  • Combined inactivated vaccine for goat mycoplasma pneumonia and preparation method of combined inactivated vaccine

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: the preparation of double inactivated vaccine of mycoplasma goat pneumoniae

[0041] 1. Preparation of mycoplasma culture medium:

[0042] (1) Preparation of pancreas extract

[0043] Take fresh pancreas from pigs that have passed the quarantine inspection, remove fat tissue, and chop; add 1500mL ddH to 500g pancreas 2 O and 500mL absolute ethanol, shake once every 2-4 hours at room temperature, and filter with filter paper after 72 hours;

[0044] (2) Preparation of Pig Lung Digestive Soup

[0045] Take the fresh pig lungs of pigs that have passed the quarantine inspection, remove the trachea and lymphatic connective tissue, chop them up, add 2000mL ddH to 1500g pig lungs 2 O, stirred and heated to 80°C, then added anhydrous NaHCO with a mass concentration of 0.8% 3 2500mL, mix well and cool to 45°C, add 500mL pancreas extract and 50mL chloroform, mix well and place at 37°C for 6-7h, then add 40mL concentrated sulfuric acid, heat at 100°C for 1h, fil...

Embodiment 2

[0058] Embodiment 2: the sterility test of the capra mycoplasma pneumonia dual inactivated vaccine

[0059] Inoculate the mycoplasma pneumoniae double inactivated vaccine prepared in Example 1 on nutrient agar medium and mycoplasma medium, at 37° C., 5% CO 2 Under culture for 3 to 7 days, no microorganisms grew.

Embodiment 3

[0060] Embodiment 3: the safety test of the double inactivated vaccine of mycoplasma goat pneumonia

[0061] (1) Material:

[0062] 2 rabbits weighing 1.5-2.0 kg.

[0063] The Mycoplasma caprico pneumonia double inactivated vaccine that embodiment 1 makes.

[0064] (2) Inspection method:

[0065] Shake the Mycoplasma caprico pneumoniae dual inactivated vaccine prepared in Example 1, inject 2 mL into each rabbit's thigh intramuscularly, raise it for 14 days under normal conditions, measure the temperature on the 1st, 5th, 10th, and 14th day after injection and Observe their food intake, mental state and whether there is any inflammatory reaction at the injection site.

[0066] (3) Test results

[0067] After the injection of the vaccine, the two rabbits had no abnormal reaction, no inflammation at the injection site, and the body temperature was 38.5-39.0°C, indicating that the prepared vaccine was safe.

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Abstract

The invention discloses a combined inactivated vaccine for goat mycoplasma pneumonia and a preparation method of the combined inactivated vaccine. The vaccine contains mycoplasma ovipneumoniae, mycoplasma mycoides subsp.capri and a propolis adjuvant. The vaccine is prepared through the following method, which comprises the following steps of 1, preparing a mycoplasma culture medium; 2, carrying out multiplication culture on mycoplasma; 3, inactivating a mycoplasma culture; and 4, mixing the inactivated mycoplasma with the propolis adjuvant to prepare the vaccine. The vaccine can be used for simultaneously preventing diseases caused by the mycoplasma mycoides subsp.capri and the mycoplasma ovipneumoniae, and avoiding the defects of single effect of a vaccine prepared through a single pathogen, is safe to goats with different physiological conditions and ages, and is suitable for goats with different varieties and various ages.

Description

technical field [0001] The invention belongs to the technical field of animal vaccines for animal husbandry, and in particular relates to a double-inactivated vaccine of mycoplasma capricum pneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma goat pneumonia refers to a contact respiratory infectious disease caused by mycoplasma in goats. Its clinical characteristics are high fever, cough, serous and fibrinous inflammation of the chest and pleura, and the acute and chronic course is taken. 22% to 30%, some as high as 60% to 80%, and the mortality rate is 15% to 30%. [0003] The prevention and control of Mycoplasma goat pneumonia is facing great difficulties, mainly because of its complex etiological factors. In addition to the prevailing dominant pathogens in my country, Mycoplasma capricum subspecies (MMC) and Mycoplasma ovis pneumoniae (MO), the pathogens of this disease that have been reported in the world include Mycoplasma capricum subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61K39/39A61P31/04C12N1/20C12R1/35
CPCA61K39/0241A61K39/39A61P31/04C12N1/20A61K2039/521A61K2039/55588A61K2039/70
Inventor 陶立李常挺吴翠兰彭昊马春霞兰美益钟舒红李军
Owner GUANGXI VETERINARY RES INST
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