Medicament for treating acute inflammation and preparation method thereof
An acute inflammation and drug technology, applied in the field of preparation of effective drugs, can solve the problems of reducing patient compliance, not dare to use the mechanism of action, and rapid onset
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Embodiment 1
[0020] Extract Group 1
[0021] Edelweiss medicinal material, add 7 times of water with a pH of 3.5 or 45% dilute ethanol, soak for 1 hour at 55°C, extract twice, filter, combine the filtrates, and recover the water or ethanol by rotary evaporation to contain 0.5% per 1ml The medicinal solution of g medicinal materials was left to stand for 48 hours to remove the precipitate, and the supernatant was placed on the macroporous adsorption resin column NKA-9, firstly eluted with 1 times the column volume of distilled water, followed by 3 times the column volume, and the volume fraction was Elute with 30% ethanol, collect the ethanol eluate, recover ethanol by rotary evaporation, and dry to dryness under reduced pressure. According to the amount of raw medicinal materials, the extracts are formulated into low, medium and high dose groups, respectively: low dose group 0.1g / ml, medium dose group 0.2g / ml, high dose group 0.4g / ml.
Embodiment 2
[0023] Extract Group 2
[0024] For edelweiss medicinal material, add 8 times of water with a pH of 4 or 50% dilute ethanol, soak for 1.5 hours at 60°C, extract 3 times, filter, combine the filtrates, and recover the water or ethanol by rotary evaporation to contain 0.5% per 1ml The medicinal solution of g medicinal materials, let it stand for 48 hours, remove the precipitate, put the supernatant on the macroporous adsorption resin column HPD500, first use 1.5 times the column volume to elute with distilled water, continue to use 4 times the column volume, the volume fraction is 40% The ethanol was eluted, the ethanol eluate was collected, the ethanol was recovered by rotary evaporation, and dried to dryness under reduced pressure. According to the amount of raw medicinal materials, the extracts are formulated into low, medium and high dose groups, respectively: low dose group 0.1g / ml, medium dose group 0.2g / ml, high dose group 0.4g / ml.
Embodiment 3
[0026] Extract Group 3
[0027] Edelweiss medicinal material, add 9 times of water with a pH of 4 or 60% dilute ethanol, soak for 2 hours at 60°C, extract 3 times, filter, combine the filtrates, and recover the water or ethanol by rotary evaporation to contain 0.5% per 1ml The medicinal solution of g medicinal materials was left to stand for 60 hours to remove the precipitate, and the supernatant was placed on the macroporous adsorption resin column X-5, and was first eluted with 2 times the volume of the column with distilled water, followed by 4 times the volume of the column, and the volume fraction was 45% ethanol was eluted, and the ethanol eluate was collected, and the ethanol was recovered by rotary evaporation, and dried to dryness under reduced pressure. According to the amount of raw medicinal materials, the extracts are formulated into low, medium and high dose groups, respectively: low dose group 0.1g / ml, medium dose group 0.2g / ml, high dose group 0.4g / ml.
[0028...
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