High-efficiency bacterial strain for degrading herbicide atrazine and function thereof

A technology of atrazine and herbicide, applied in the field of chemical pesticide biodegradation, can solve the problems of reduced energy utilization rate, insufficient oxygen supply to cells, attenuation, etc.

Inactive Publication Date: 2010-06-16
谢明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Atrazine can inhibit erythropoiesis in Rana adenopleura tadpoles, resulting in insufficient oxygen supply to the cells, lower energy utilization rate, further inhibit the growth of tadpoles, and delay development. The individual size during metamorphosis will affect the predation ability of adults, which may be through This pathway causes the decline of such populations

Method used

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  • High-efficiency bacterial strain for degrading herbicide atrazine and function thereof
  • High-efficiency bacterial strain for degrading herbicide atrazine and function thereof
  • High-efficiency bacterial strain for degrading herbicide atrazine and function thereof

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Experimental program
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Effect test

Embodiment 1

[0115] Embodiment 1: The impact of environmental factors on the degradation efficiency of bacterial strains. When the concentration of atrazine is low (200mg / L), the strain can completely degrade it (100%) within 3 days, and the time for complete degradation is correspondingly significantly extended as the concentration of atrazine increases. With the increase of the concentration of inoculated bacteria in the medium, the degradation rate of atrazine also gradually increased. 6 cfu / ml increased to 3.28×10 8When cfu / ml, the corresponding degradation rate increased from 54.0% to 99.7%. In the inorganic salt liquid medium, the degradation rate was high in weakly acidic and neutral environments (pH 5, 6, 7) and weakly alkaline (pH 8, 9, 10), and the optimum pH for degradation was 7. Within the temperature range of 15-40°C, the degradation rate varies from 88% to 99%.

Embodiment 2

[0116] Example 2: Basic conditions for strain growth. When the strain grows on the beef extract peptone medium, it is in the delayed growth phase from 0 to 6 hours, in the logarithmic growth phase from 6 to 20 hours, and enters the stable and decay phase after 20 hours. It can grow under the condition of 45°C, and the optimum growth temperature is 30°C; the suitable pH of the strain is between 6 and 9, and the optimum pH is 7; the growth of the strain does not require much oxygen.

Embodiment 3

[0117] Example 3: Degradation dynamics of strains to atrazine. The strain was inoculated under the condition of inorganic salt liquid medium with atrazine as the only nitrogen source, when the bacterial cell concentration was 6.59×10 8 When cfu / ml, bacterial strain can reach 89.5% to the degradation rate of atrazine in the first 3 days, and the degradation rate can reach 100% on the 7th day (atrazine is not detected in the medium, and the detection limit is 0.01ng / μl). Degradation rate% = (residual pesticide amount in the system after cultivation / initial pesticide addition amount) × 100%

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Abstract

The invention discloses a high-efficiency bacterial strain for degrading herbicide atrazine and a function thereof, which belong to the technical field of biodegradation for chemical herbicides, and relate to an indigenous bacterial strain and the degradation of the herbicide atrazine thereof. The bacterial strain is a shinella sp.HBZA0511 bacterial strain, which is preserved in the Centre of General Microbiology of China Committee for Culture Collection of Microorganisms (CGMAA), has a preserving number of CGMCC No.1801, and can be used for in-situ quick fix engineering for herbicide pollution of soil.

Description

technical field [0001] The invention belongs to the technical field of biodegradation of chemical pesticides, and relates to a native bacterium Shinella sp. HBZA0511 strain (Graninella sp.) and its degradation effect on the pesticide atrazine, which can be used in the in-situ restoration project of soil pesticide pollution. Background technique [0002] Atrazine (Atrazine, chemical name 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, molecular formula C 8 h 14 ClN 5 ), also known as atrazine, is a s-triazine herbicide and is widely used in corn fields, sorghum fields, sugarcane fields, orchards, nurseries, and woodlands to prevent and control annual grass weeds, and also has a certain inhibitory effect on perennial weeds . Atrazine can be used before or after emergence, but it has a long residual period in the soil and has biological activity, which is easy to cause phytotoxicity to some sensitive crops such as wheat, soybean, rice, etc.; atrazine Razin has good w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10A62D101/04C12R1/01
Inventor 谢明万方浩应飞邱卫亮
Owner 谢明
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