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Culture medium for in vitro culture of epidermal melanophore

A melanocyte, in vitro culture technology, applied in artificial cell constructs, animal cells, vertebrate cells, etc.

Active Publication Date: 2013-04-10
许爱娥 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of metaproterenol on epidermal melanocyte growth and melanin synthesis has not been reported yet

Method used

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  • Culture medium for in vitro culture of epidermal melanophore
  • Culture medium for in vitro culture of epidermal melanophore
  • Culture medium for in vitro culture of epidermal melanophore

Examples

Experimental program
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Effect test

Embodiment 1

[0057] 1.1 Materials and methods

[0058] 1.1.1 Preparation of medium

[0059] Add glutamine to Ham's F12 (GIBCO, Carlsbad, Calif.) to a final concentration of 2 mM; gentamicin to a final concentration of 50 μg / ml; fetal bovine serum (FBS, GIBCO, Carlsbad, Calif.) to a final concentration of 10 % (volume / volume), the culture medium A containing FBS and gentamicin was obtained, stored at 4 degrees, and freshly prepared once every two weeks.

[0060]bFGF (PeproTech, Rocky Hill, N.J.) was dissolved in F12 medium at a concentration of 2500 ng / ml, and stored at -70°C after aliquoting. Once a week, the bFGF stock solution was added to medium A to a final concentration of 25 ng / ml.

[0061] FGF20 (PeproTech, Rocky Hill, N.J.) was dissolved in F12 medium at a concentration of 3000ng / ml, and stored at -70°C after aliquoting. Once a week, FGF20 stock solution was added to medium A to a final concentration of 30 ng / ml.

[0062] IBMX powder (Sigma, St.Louis, Mo.) was dissolved in cell...

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Abstract

The invention provides a culture medium for in vitro culture of epidermal melanophore. In the culture medium, a basic culture medium which is suitable for in vitro culture of the epidermal melanophore is used as a substrate, and growth factors (1), cAMP accelerant (2) and serum (3) are added into the substrate. The invention has the beneficial effects that the invention provides the culture medium which is specially used for in vitro culture of the epidermal melanophore, and the culture medium can be used for culturing epidermal melanophore with strong propagation capacity, migration capacityand melanin synthesizing capacity, can enable melanophore of patients to better grow and differentiate in vitro, and can be used for transplantation treatment of depigmented or nonpigmented dermopathic patients.

Description

(1) Technical field [0001] The invention relates to a medium for culturing epidermal melanocytes in vitro. (2) Background technology [0002] Skin color is primarily determined by the combination of pigments manufactured by the skin and natural pigments contained in the top layers of the skin. The epidermis on the surface of the epithelial layer of the skin contains epidermal melanocytes that produce skin pigment (melanin). In people with hypopigmented or amelanotic skin conditions, the melanocytes in the skin are missing, damaged, or nonfunctional, resulting in white spots on various parts of the body. [0003] Melanin is not only protective, it is also crucial for skin aesthetics. Some diseases can cause local melanocyte destruction, loss or inhibition of cell function, affecting the skin pigment production system, resulting in skin hypopigmentation or loss of pigmentation. Such abnormalities can cause serious psychological and sociological damage to the patient. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071
Inventor 许爱娥胡诞宁傅丽芳周妙妮尉晓冬洪为松关翠萍
Owner 许爱娥