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Aroma gene in rice and functional marker thereof

A fragrance gene and rice technology, applied in genetic engineering, plant genetic improvement, biochemical equipment and methods, etc.

Inactive Publication Date: 2010-06-16
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many scholars have also developed corresponding molecular markers based on the existing rice aroma genes, but although some aroma rice has aroma, the aroma gene sequence is not consistent with the above research, which will provide opportunities for the discovery and utilization of new aroma genes

Method used

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  • Aroma gene in rice and functional marker thereof
  • Aroma gene in rice and functional marker thereof
  • Aroma gene in rice and functional marker thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: A method for obtaining a new deletion site of rice aroma gene:

[0018] 1. Extraction of genomic DNA from leaves of local aromatic rice varieties in Miaoxiangnuo

[0019] The DNA extraction method mainly refers to the method of Lu Yangjiang and Zheng Kangle (1992). The specific method is as follows: put about 2-3 cm of fresh leaves into a mortar, add 400 μL of DNA extraction solution, grind until the leaves are ground, and then add 400 μL of DNA extraction solution , mix well; add 500 μL of chloroform extract, close the lid tightly, and mix upside down; centrifuge at 11000 rpm / min for 1 minute until the phases are clearly separated; transfer 400 μL of the supernatant to a new labeled 1.5mL centrifuge tube; add 800 μL of pre-cooled absolute ethanol, tightly cover the lid, mix up and down, centrifuge at 12000 rpm / min for 3 minutes until the precipitate is attached to the bottom of the centrifuge tube, and discard the supernatant. Wash the precipitate with 7...

Embodiment 2

[0053] Example 2: Development of rice aroma gene functional markers and detection of aroma genes in some aromatic rice materials

[0054] 1. Test materials

[0055] The aromatic rice materials studied in this experiment are listed in Table 2, including 22 aromatic rice materials and 4 non-aromatic rice materials.

[0056] Table 2 4 non-fragrant rice and 22 fragrant rice materials studied in this experiment

[0057]

[0058] Note: - indicates non-fragrance material; + indicates fragrance material

[0059] 2. Aroma identification of 22 fragrant rice and 4 non-fragrant rice materials

[0060] Flavor identification method mastication method and potassium hydroxide method are the same as described in Example 1.

[0061] The results showed that 22 aromatic rice seeds and leaves showed fragrance, and 4 non-aromatic rice materials had no fragrance.

[0062] 3. DNA extraction, PCR amplification analysis

[0063] The DNA extraction method and PCR amplification analysis are as sh...

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Abstract

An aroma gene in rice and a functional marker thereof belong to the biotechnology field. The aroma gene is characterized in that the aroma gene is allelic to a coded betaine-aldehyde dehydrogenase gene Badh2 on chromosome 8; compared with the normal Badh2, in the aroma gene, 803bp is deleted between the fourth exon and the fifth exon; the deleted base is positioned between downstream 1628bp and 2430bp of a transcription initiator codon of the gene; and the 803bp deleted base corresponds to the fourth exon 90bp, the fourth intron 694bp and the fifth exon 19bp. The functional marker FMbadh2-E4-5 of the aroma gene is designed according to the aroma gene in rice. By the method, the aroma gene can be transplanted to the non-aroma rice varieties with target, thus greatly promoting cultivation of new high-quality aromatic rice varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the development of a rice aroma gene and its functional marker. Background technique [0002] Fragrant rice is very popular because of its unique fragrance characteristics, and the price of fragrant rice in the market is also much higher than that of non-fragrant rice. Therefore, breeding of fragrant rice has become one of the important contents of modern rice breeding. The molecular biological mechanism of inheritance is beneficial to the selection and breeding of new varieties. [0003] So far, the study of aroma genes in rice has made great progress, and many scholars have carried out genetic analysis and gene mapping of aroma genes in rice. Ahn et al. (1992) first used restriction fragment length polymorphism markers to locate the recessive gene controlling fragrance on the eighth chromosome, and the genetic distance from RFLP marker RG28 was 4.5cM. Lorieux et al. (...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12Q1/68
Inventor 胡培松唐绍清邵高能罗炬焦桂爱魏详进唐傲
Owner CHINA NAT RICE RES INST
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